Thermo Fisher, P1267MP, PBFI, AM, cell permeant - Special Packaging

PBFI, AM, cell permeant - Special Packaging PBFI is a potassium-sensitive molecule used measure intracellular K+fluxes in animal cells and in plant cells and vacuoles. Although the selectivity of PBFI for K+is less than that of calcium indicators such as fura-2, it is sufficient for the detection of physiological concentrations of K+in the presence of other monovalent cations. The spectral response of PBFI upon ion binding permit excitation ratio measurements, and this indicator can be used with the same optical filters and equipment used for fura-2.
Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›Fluorescent Ion Indicators Specifications:
• Label (Ex/Em): PBFI (∼340,380/500 nm)
• Lyophilized product may be dissolved in DMSO for use
• Product is typically loaded into cells by adding the dissolved indicator to medium containing cellsSelectivity Considerations and Cell Loading StrategiesThe Kdof PBFI for K+is strongly dependent on whether Na+is present, with a value of 5.
1 mM in the absence of Na+and 44 mM in solutions with a combined Na+and K+concentration of 135 mM (which approximates physiological ionic strength). In buffers in which the Na+is replaced by tetramethylammonium chloride, the Kdof PBFI for K+is 11 mM; choline chloride and N-methylglucamine are two other possible replacements for Na+in the medium. Although PBFI is only 1.
5-fold more selective for K+than for Na+, this selectivity is often sufficient because intracellular K+concentrations are normally about 10 times higher than Na+concentrations.
PBFI is available as both cell-impermeant acid salt (P1265MP) and as cell-permeant acetoxymethyl (AM) esters (P1267MP). The anionic acid forms can be loaded into cells using our Influx™ pinocytic cell-loading reagent (I14402,Chelators, Calibration Buffers, Ionophores and Cell-Loading Reagents—Section 19.
8), or by microinjection, patch-pipette infusion or electroporation. For AM ester loading (Loading and Calibration of Intracellular Ion Indicators—Note 19.
1), addition of the Pluronic™ F-127 (P3000MP,P6866,P6867) or PowerLoad™ (P10020) dispersing agents as well as relatively long incubation times—up to four hours—are typically necessary.
Find Fluorescent Indicators for Na+and K+We offer a number of fluorescent indicators for measuring Na+and K+. ReviewFluorescent Na+ and K+ Indicators—Section 21.
1in the Molecular Probes™ Handbook for more information on these products.
For Research Use. Not for human or animal therapeutic or diagnostic use.
Detection Method: Fluorescence
Dye Type: Potassium Indicator
Quantity: 20 x 50 μg
Shipping Condition: Room Temperature
For Use With (Application): Cell Viability and Proliferation
For Use With (Equipment): Fluorescence Microscope
Product Type: PBFI AM
Unit Size: Each