Thermo Fisher, PQ1000101, ProQuest™ Two-Hybrid System with Gateway™ Technology

Thermo Fisher, PQ1000101, ProQuest™ Two-Hybrid System with Gateway™ Technology The ProQuest™ Two-Hybrid System with Gateway™ Technology is anin vivoyeast-based system for identifying interactions between two proteins. This interaction reconstitutes a functional transcription factor that activates chromosomally integrated reporter genes where transcriptional activation is monitored by the growth of cells on selective media.
The ProQuest™ System features:
• Gateway™ Technology to allow rapid and easy generation of bait and prey constructs, and to facilitate downstream application
• Low copy-number DBD (DNA Binding Domain) and AD (Activation Domain) vectors (ARS/CEN) to control over-expression and increase reproducibility
• Three different reporter genes (HIS3,URA3, andlacZ) with independent promoter regions to rapidly weed out false positives.
URA3 reporter gene allows for both positive and negative selection, enabling advanced two-hybrid techniques such as reverse two-hybrid.
• A set of strong-to-weak Gateway™-based control interactions to evaluate results and assess the strength of the interaction being testedAssay: Two-Hybrid Assay
Expression System: Yeast
Product Type: Two-Hybrid System
Quantity: 1 kit
Reporter Gene: Beta-Gal (lacZ), HIS3, URA3
Product Line: Gateway™, ProQuest™
Unit Size: Each