Thermo Fisher, PV3176, Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon bovine MBP Thr-97 derived peptide, containing a P-X-(S/T)-P consensus sequence, where X is any residue. Assay: Serine/Threonine Kinase Assay
Assay Entry: Biochemical phospho-specific activity (no antibody)
Excitation/Emission: 405/465, 535 nm
For Use With (Application): FRET
Format: 384-well Plate
Label or Dye: Methylcoumarin
No. of Reactions: 800 x 20 μL Reactions
Quantity: 800 x 20 μL Assays
Readout: End Point
References: BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978)
Shipping Condition: Dry Ice
Source: Bovine MBP, Thr-97 Derived Peptide S5, Consensus Seq Pro-X-(Ser⁄Thr)-Pro (APRTPGGRR)
Substrate Properties: Peptide-Based Substrate
Target Entry: MAPK13, MAP2K1, MAP2K6, MAPK1, MAP3K8, BRAF, MAP2K2, MAPK12, MAPK3, BRAF V599E
Target Enzyme: Serine/Threonine Kinases
Validated Kinases: BRAF, MAPK1, MAPK3, MAPK12, MAPK13, MAP2K1, MAP2K2, MAP3K6, MAP2K8
Product Line: Z ́-LYTE™
Unit Size: Each