Thermo Fisher, PV5830, Kinase Tracer 199, 25 μM

Kinase Tracer 199, 25 μM In the LanthaScreen® Eu Kinase Binding Assay, binding of an Alexa Fluor® conjugate or 'tracer' such as Kinase Tracer 199 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps. Visit www.
invitrogen.
com/bindingassay to learn more and to see which kinases have been validated using this kinase tracer.
Assay: Kinase Binding Assay
Assay Entry: Biochemical binding/kinetic
Color: Far-Red
Excitation/Emission: 340/665
For Use With (Application): TR-FRET
Label or Dye: Alexa Fluor™ 647
Quantity: 25 μL
Shipping Condition: Dry Ice
Target Entry: MAPK8, EGFR, RIPK2, MAPK9, EGFR T790M L858R, MAPK14, MAPK10, EGFR L861Q, EGFR L858R
Validated Kinases: EGFR T790M L858R, EPHB4, KIT V654A, MAPK9 (JNK2), RIPK2
Detection Method: Fluorescence
Type: Kinase Tracer
Unit Size: Each