Thermo Fisher, R37106, NucRed™ Live 647 ReadyProbes™ Reagent

NucRed™ Live 647 ReadyProbes™ Reagent NucRed Live 647 ReadyProbes Reagent is a bright, far-red, cell-permeant nuclear stain for live or fixed cells. It is provided as a room temperature-stable solution in a convenient-to-use dropper bottle. Just add two drops per ml to stain your cells. Provided at 12. 5X concentration and formulated with 1% DMSO, PowerLoad solubilizing agent, and HBSS buffer. Also available asHCS Nuclear Mask Deep Red Stainat 250X concentration in DMSO.
• No need to dilute, weigh, or pipette
• Convenient dropper bottle—just use two drops per mL
• Stable at room temperature—keep handy at your scope or cell culture area
• Excited at 638 nm when bound to DNA, with an emission maximum at 686 nmSee other ReadyProbes reagents for cell stainingSee other nuclear stains for imagingCell imaging applicationsThe far-red fluorescence emission makes it ideal for use in combination with blue, green, and red fluorophores, such as Hoechst, GFP/FITC, and Texas Red. Suggestions for use
• NucRed Live 647 ReadyProbes Reagent may be added directly to cells in full media, or buffer solutions.
• In most cases, 2 drops/mL and an incubation of 15 to 30 minutes will give bright nuclear staining; however, more or fewer drops can be added for optimal staining intensity.
• In most cases, staining intensity increases with time if cells are not washed prior to imaging.
• It is detected through a far-red filter, such as a Cy5 filter, or a Cy5 Light Cube for EVOS imaging systems. Visualize staining your cell without wasting your reagents, antibodies, or time with our newStain-iT Cell Staining Simulator. Color: Far-Red
Detection Method: Fluorescence
Dye Type: Cell-Permeant
Emission: Visible
Excitation Wavelength Range: 638⁄686
For Use With (Equipment): Confocal Microscope, Fluorescence Microscope, Flow Cytometer
Form: Liquid
Product Line: NucRed™, ReadyProbes™
Quantity: 6 dropper bottle(s) kit
Label Type: Fluorescent Dye
Product Type: Nucleic Acid Stain
SubCellular Localization: Nucleus
Unit Size: Each