Thermo Fisher, R37111, CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent

Thermo Fisher, R37111, CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent is a fluorogenic, no-wash indicator of activated caspase-3/7 for live- and fixed-cell applications. Activation of caspase-3 is an early indicator of apoptosis and CellEvent™ Caspase-3/7 Green reagent allows rapid and sensitive detection of cells destined for cell death.
• Simple and fast no-wash protocol helps preserve delicate apoptotic cells
• Compatible with both live-cell fluorescence imaging and formaldehyde-based fixation methods
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stability at room temperature—keep handy at your scope or cell culture area.
See other ReadyProbes™ reagents for cell stainingLearn more about other assays for apoptosisCell imaging applicationsCellEvent™ Caspase-3/7 Green reagent is a four amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye that is non-fluorescent when not bound to DNA. The CellEvent™ Caspase-3/7 Green reagent is intrinsically non-fluorescent, as the DEVD peptide inhibits binding of the dye to DNA. Upon activation of caspase-3/7 in apoptotic cells, the DEVD peptide is cleaved and the free dye can bind DNA, generating a bright green fluorescence. The fluorescence emission of the dye when bound to DNA is 530 nm and can be observed using a standard FITC filter set.
Suggestions for use
• In most cases, 2 drops/ml and an incubation time of 30 minutes is sufficient for bright nuclear staining of apoptotic cells; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• If desired, combine CellEvent™ Caspase-3/7 Green ReadyProbes™ reagent with a red or far-red nuclear stain for a total cell count
• CellEvent™ Caspase-3/7 Green dye is excited with a maximum at 502 nm and has an emission maximum at 530 nm. It is detected through standard GFP and FITC filters.
Color: Green
Compatible Cells: Mammalian Cells
Excitation/Emission: 502/530
For Use With (Application): Cell Imaging
For Use With (Equipment): Confocal Microscope, Floid™ Cell Imaging System, Fluorescence Microscope, Flow Cytometer, Floid™ Cell Imaging System
Label Type: Other Label(s) or Dye(s)
Product Line: CellEvent™, ReadyProbes™
Product Type: Caspase 3/7 Reagent
Quantity: 1.
75 mL kit
Detection Method: Fluorescence
Format: Dropper Bottle
Unit Size: Each