Thermo Fisher, S11223, Streptavidin, Alexa Fluor™ 488 Conjugate

Solid, 1 mg Alexa Fluor™ 488 streptavidincomprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0. 5 mL volumes of a 2 mg/mL solution. Important Features of Alexa Fluor™ 488 Streptavidin Conjugates:
•Alexa Fluor™ 488 streptavidin conjugate has Ex/Em maxima of ∼ (495/519)
•Bright, photostable fluorescence
•High solubility in aqueous solutions
•Available in multiple colors
•Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and moreProperties of Alexa Fluor™ DyesAlexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications. Blocking Endogenous BiotinNaturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit (Cat. No. E21390) to minimize this interference. See User Manual for solubility instructions. Excitation/Emission: 495/519 nm
Label or Dye: Alexa Fluor
Product Type: Streptavidin Conjugate (fluorescent)
Quantity: 1 mg
Shipping Condition: Room Temperature
Conjugate: Alexa Fluor 488
Form: Solid
Product Line: Alexa Fluor™
Unit Size: Each