Thermo Fisher, V23201, Chromatin Condensation & Membrane Permeability Dead Cell Apoptosis Kit with Hoechst 33342, YO-PRO™-1, and PI dyes, for flow cytometry

Chromatin Condensation & Membrane Permeability Dead Cell Apoptosis Kit with Hoechst 33342, YO-PRO™-1, and PI dyes, for flow cytometry This product detects apoptotic cells with changes in nuclear chromatin condensation and plasma membrane permeability, using three nucleic acid stains: UV-excitable Hoechst 33342, green fluorescent YO-PRO™ dye, and propidium iodide. The YO-PRO™ dye can enter apoptotic cells, whereas red-fluorescent propidium iodide (PI) cannot. Thus after staining with YO-PRO™-1 dye and PI, apoptotic cells show green fluorescence, and dead cells show primarily red fluorescence and some green fluorescence. Blue fluorescent Hoechst 33342 brightly stains the condensed chromatin of apoptotic cells and more dimly stains the normal chromatin of live cells. The staining pattern resulting from the simultaneous use of these three dyes makes it possible to distinguish normal, apoptotic and dead cell populations by flow cytometry or fluorescence microscopy.
Excitation/Emission: YO-PRO™-1: 491/509 cm, Hoechst 33342: 350/461 cm, PI: 535/617 cm
Flow Cytometer Laser Lines: 488 nm
For Use With (Application): Flow Cytometry
For Use With (Equipment): Flow Cytometer
Form: Solution
No. of Reactions: 200 Reactions
Product Line: Vybrant™, YO-PRO™
Product Type: Dead Cell Apoptosis Kit
Quantity: 1 kit
Shipping Condition: Room Temperature
Conjugate: Hoechst 33342, Propidium Iodide, YO-PRO-1
Format: Kit
Unit Size: 1 kit