Thermo Fisher, V35118, Vybrant™ FLICA Caspase Apoptosis Assay Kits for flow cytometry

FAM, Hoechst 33342, propidium iodide, Vybrant FAM Caspase-3 and -7 Assay Kit Quickly detect caspase-mediated apoptosis by flow cytometry with Vybrant FAM caspase assay kits for poly-caspases and caspases 3 and 7. The Vybrant FAM caspase assay kits use fluorescent inhibitor of caspases (FLICA) methodology to detect and report caspase activity. Specifically, these caspase assay kits contain engineered FLICA substrates FAM-VAD-FMK (for poly-caspases), FAM-DEVD-FMK (for caspase-3/7), and FAM-LETD-FMK (for caspase-8). They also include Hoechst 33342 and propidium iodide stains, enabling simultaneous evaluation of membrane permeability and cell cycle by flow cytometry.
Description: Vybrant FAM Caspase-3 and -7 Assay Kit
Excitation/Emission: FAM-DEVD-FMD: 388/530PI: 535/617Hoechst 33342: 350/461
Flow Cytometer Laser Lines: UV, 488
For Use With (Equipment): Flow Cytometer
Kit Contents: 1 vial FAM-DEVD-FMK caspase-3 and -7 reagent (FLICA reagent, lyophilized solid), 1 vial of Hoechst 33342 (400 μL, 1 mM in water), 1 vial of PI (1 mL, 250 μg/mL in water), 1 vial of DMSO (0.
5 mL), 1 bottle of apoptosis fixative solution (10% formaldehyde with methanol in PBS, 6 mL), and 1 bottle of 10x apoptosis wash buffer.
Label Type: Other Label(s) or Dye(s)
Label or Dye: FAM, Hoechst 33342, Propidium Iodide
Product Line: Vybrant
Product Type: Caspase Assay Kit
Quantity: 25 assays
Shipping Condition: Room Temperature
Storage Requirements: Store in refrigerator (2–8°C) and protect from light.
Detection Method: Fluorescence
Format: Tube
Unit Size: 25 assays