Waters, 176003595, XBridge Protein BEH SEC Column, 200Å, 3.5 µm, 7.8 mm X 150 mm, 1/pk and BEH200 SEC Protein Standard Mix

XBridge Protein BEH SEC, 200 Å Columns, containing 3.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 10,000 – 450,000 Daltons. Note: This P/N also contains one vial the BEH200 SEC Protein Std (P/N 186006518) at no additional cost.

Specifications
Chemistry: SEC
Separation Mode: Gel Filtration (aqueous)
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 3200 psi (221 Bar)
Endcapped: No
Bonding Technology: Diol (OH)
Silanol Activity: Low
Molecular Weight Range Min: 10000
Molecular Weight Range Max: 450000
Particle Shape: Spherical
Particle Size: 3.5 µm
Endfitting Type: Parker-style
Pore Size: 200 Å
QC Tested: Protein
Format: Online Column
Packing Solvent: 20% methanol in water
Surface Area: 220
System: HPLC
Particle Technology: BEH
Technique: LC
USP Classification: L33
Inner Diameter: 7.8 mm
Length: 150 mm
Carbon Load: 12 %
eCord: No
UNSPSC: 41115711
Application: Protein
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge Protein BEH SEC Column, 200Å, 3.5 µm, 7.8 mm X 150 mm, 1/pk and BEH200 SEC Protein Standard Mix Compared to other conventional columns in the market, the XBridge Protein BEH SEC Columns, with a 200 Å pore size, containing 3.5um particles, are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used for the HPLC-based SEC columns. In contrast to the silica-based SEC Columns, the XBridge Protein BEH SEC Column offers fewer non-desired protein and column interactions, which enables the column to deliver exceptional column life. They are best suited to separate proteins ranging from 10,000 – 450,000 Daltons. The diol-bonded coating provides the XBridge Protein BEH SEC Column flow and pressure tolerance for increased sample throughput on HPLC systems. This combination of BEH base particle technology with diol bonding processes enables it to deliver column stability, performance, and increased lifetime. In this listing, you will also obtain the BEH200 SEC Protein Standard Mix; it is a comprehensive protein mixture containing five components: Thyroglobulin, IgG, BSA, Myoglobin, and Uracil. This standard is recommended for benchmarking column and system performance while running your tests. You can use it to benchmark ACQUITY UPLC BEH200 SEC, 1.7 µm Columns, and the XBridge Protein BEH SEC Column as well. By using the XBridge Protein BEH SEC Column, you will get seamless migration to UltraPerformance LC Technology, and this equipment is designed to deliver maximized productivity. Similar to all equipment manufactured by Waters, the XBridge Protein BEH SEC Columns are reliable and guarantee reproducibility as they are made in cGMP, ISO9001, and ISO13485 certified facilities. Each piece of equipment goes through rigorous testing at every stage and phase of production to give the highest quality equipment you can rely on and count on. The column is an essential piece of lab equipment as it provides unmatched column lifetime and the flexibility to work over an unparalleled range of mobile phase pH and temperatures. Shop for lab equipment through the catalog on our website and find our complete range of XBridge columns available for use across a wide range of applications. Please feel free to reach out to a member of our global team, and we would be happy to assist you with any queries. You may also be interested in XBridge Protein BEH SEC Guard Column, 200Å, 3.5 µm, 7.8 mm X 30 mm , which is manufactured using the same packing materials as the analytical column and designed exclusively to protect your column from harm.

FAQ
What Is The Optimal pH Range For The XBridge Protein BEH 200 SEC Column? The XBridge Protein BEH 200 SEC Column ideally operates within the 2-8 pH range. Please keep in mind, using higher pH may impact the performance and lifetime of your column.