BD, 554213, BD Pharmingen™ Purified Mouse Anti-Human E2F-1

Reactivity: Human (QC Testing), Mouse, Rat (Tested in Development)
Isotype: Mouse IgG2a
Immunogen: Human E2F-1 recombinant protein aa. 1-437
Application: Western blot (Routinely Tested), Immunoprecipitation (Reported)
Target Molecular Weight: 60 kDa
Concentration: 0.5 mg/ml
RRID: AB_395308
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Applications include western blot analysis (dilute antibody 1-2 µg/ml). MOLT-4 leukemia cells (ATCC CRL-1582), Daudi lymphoma cells (ATCC CCL-213), and RS4; 11 leukemia cells (ATCC CRL-1873) are suggested as positive controls. Other applications include immunoprecipitation (1-2 µg/1x10^6 cells).  For western blot, detailed protocol is available at: http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml