BD, 554263, BD Pharmingen™ Purified Mouse Anti-Mouse TFE3

Reactivity: Human (QC Testing), Mouse (Tested in Development)
Isotype: Mouse IgG1
Immunogen: TFE3-L Protein
Application: Western blot (Routinely Tested), Gel shift, Immunoprecipitation (Reported)
Target Molecular Weight: 50 kDa
Concentration: 0.5 mg/ml
RRID: AB_395332
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Applications include western blot analysis (0.25-1.0 µg/ml). WI-38 cells are suggested as a positive control. Other applications include gel shift (supershifts) and immunoprecipitation, which are not routinely tested by BD Biosciences Pharmingen. The antibody has been used to detect in vitro translated TFE3-L and TFE3-L, expressed as a recombinant protein in bacteria. In gel shift assays, using µE3 as a probe, this antibody supershifts the complex.