BD, 558739, BD Pharmingen™ Purified Rat Anti-Mouse CD44

Alternative Name: Pgp-1, H-CAM, Ly-24
Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
Immunogen: (C57BL/6 x DBA/2)F1 mouse bone marrow-derived stromal cell clone BMS2
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Western blot (Tested During Development), Blocking, ELISA (Reported)
Concentration: 0.5 mg/ml
RRID: AB_397098
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures For quantitation of soluble CD44, a sandwich ELISA using purified IM7 antibody (Cat. no. 553131) for capture and biotinylated KM114 mAb for detection has been described. For IHC, we recommend the use of purified IM7 mAb in our special formulation for immunohistochemistry, Cat. no. 550538.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.