Cytiva, 17544123, HiTrap Capto™ S cation exchange chromatography column

HiTrap Capto S, 5 × 5 mL HiTrap Capto S chromatography column is prepacked with a strong cation exchange modern resin for small scale protein purification. • Suitable for screening of selectivity, binding and elution conditions, and small-scale purifications. • Sulfoethyl (S) strong cation exchanger. • Optimized for capture and intermediate protein purification. • Convenient HiTrap format for easy connection to a syringe, peristaltic pump, or chromatography system such as an ÄKTA system. Protein capture and intermediate purification Capto S resin couples a sulfoethyl strong cation exchanger to a chemically modified, high-flow agarose matrix. The rigid agarose matrix provides greater particle rigidity without compromising pore size, which allows for high bed volume and purification of viscous samples at high flow rates. High dynamic binding capacity is achieved with a dextran surface extender that coats the agarose matrix. The resin’s high binding capacity, high flow rate, and low back pressure reduce process cycle times and improve productivity at process scale. Successful use in published research Applications for these strong cation exchange chromatography columns include: Analysis of the binding properties of recombinant Plasmodium falciparum VAR2CSA Purification of a new actinoporin Gixantoxin-4 Purification and characterization of the staphylococcal bacteriophage lysin CHAP(K) Other Capto S resin formats Capto S resin is also available as bulk resin and in other prepacked formats. Therefore, it is possible to use the same resin for development work, pilot studies, and routine production. Other Cytiva ion exchange products Cytiva's solutions include a variety of ion exchange chromatography resins, such as Capto, Sepharose, and SOURCE.