Qiagen, 51206, FlexiGene DNA Kit (250)

For isolation of genomic DNA from whole blood, buffy coat, and cultured cells in a single tube with fast kit preparation

Features

- Easy handling, no risk of sample mix-up
- Scalable purification from 0.1–20 ml whole blood
- Direct purification from whole blood, buffy coat, and cultured cells
- Fast procedure
- No organic extraction

Product Details

The FlexiGene system uses simple centrifugation to isolate highly pure DNA from whole blood, buffy coat, and cultured cells. The FlexiGene DNA Kit contains buffers and QIAGEN Protease for direct, simple purification in a single tube. For automated solutions using FlexiGene reagents, contact Autogen, Inc.

Performance

Sample: Volume
Blood*: 0.3 ml
Blood*: 2.0 ml
Blood*: 10.0 ml
Buffy coat: 0.5 ml
HeLa cells: 10 6 cells

DNA purified using the FlexiGene system is sized up to 150 kb (see figure " High-quality genomic DNA "). It performs well in amplification and Southern blotting experiments, and is also suitable for archiving (see figure " Efficient downstream PCR analysis ").

DNA is purified from 0.1–20 ml (blood), 0.1–2 ml (buffy coat), or 1–2 x 10 6 cells (cultured cells) in a single tube, processed by centrifugation. Twelve samples can be processed in 1 hour. Depending on sample size, the rehydration volume is 0.2–1 ml.

Principle

The FlexiGene DNA system enables isolation of genomic DNA from variable amounts of whole blood, buffy coat, and cultured cells. The simple purification procedure is performed in a single tube, which reduces costs and simplifies handling. The purified DNA performs well in sensitive downstream applications.

Applications

- PCR
- Southern blotting
- Archiving

DNA purified using the FlexiGene DNA kit performs well downstream applications, including,