Waters, 186001301, Atlantis dC18 Column, 100Å, 5 µm, 2.1 mm X 150 mm, 1/pk

Atlantis dC18 columns are universal, silica-based, reversed-phase C18 columns that are used for polar compound retention. The Atlantis dC18 columns feature di-functionally bonded C18 ligands that have been optimized for use with highly aqueous mobile phases, including 100% water.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Silica
pH Range Min: 3 pH
pH Range Max: 7 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: Yes
Bonding Technology: dC18
Silanol Activity: Medium
Particle Shape: Spherical
Particle Size: 5 µm
Endfitting Type: Waters
Pore Size: 100 Å
Format: Column
Surface Area: 330
System: HPLC
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 150 mm
Carbon Load: 12 %
UNSPSC: 41115709
Brand: Atlantis
Product Type: Columns
Units per Package: 1 pk

Additional Information
FAQs about Atlantis dC18 Column What applications are best served by the Atlantis dC18 Column? The Atlantis dC18 Column is designed for the reversed-phase chromatography of polar and non-polar compounds, making it suitable for a wide range of applications, including pharmaceuticals, natural products, and environmental samples. How does the Atlantis dC18 Column enhance retention of polar analytes? The column's unique bonding chemistry is optimized to enhance the retention of polar analytes, which can be challenging to retain on traditional C18 columns. Is the Atlantis dC18 Column compatible with high pH mobile phases? Yes, the Atlantis dC18 Column offers high pH stability, allowing for the use of mobile phases with higher pH values, which can improve the separation of certain analytes. What is the significance of the 100Å pore size in the Atlantis dC18 Column? The 100Å pore size is suitable for small molecule separations, providing a balance between surface area and access, which can be particularly beneficial for the separation of small to medium-sized polar compounds. What are the guidelines for cleaning and storing the Atlantis dC18 Column? To ensure the longevity of the Atlantis dC18 Column, it should be flushed with a mixture of water and organic solvents post-analysis and stored in accordance with the manufacturer's recommendations, typically in a solvent that will not cause phase collapse or degradation.

FAQ
How Are Atlantis dC18 Columns Optimized For Aqueous Mobile Phases? In conventional reversed-phase HPLC, columns often have difficulty retaining and separating highly polar, water-soluble compounds. Retaining these analytes can require the use of mobile phases with little to no organic modifier, and C18 stationary phases may experience a sudden loss of retention due to a “collapse” of C18 chains.
Waters has come to determine that silica pores actually expel aqueous mobile phases in the absence of pressure. This causes analytes to not migrate into the pores, passing through the column unretained- known as “Dewetting.” Atlantis dC18 Columns were developed specifically to counteract dewetting and operate in aqueous mobile phases.