Waters, 186003613, XBridge Peptide BEH C18 Column, 300Å, 3.5 µm, 4.6 mm X 150 mm, 1K - 15K, 1/pk

The XBridge BEH300 C18 Peptide Separation Technology (PST) columns are optimized and QC tested for peptide separations. The wide-pore (300Å) trifunctionally bonded BEH particle offers you the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 12 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: Yes
Silanol Activity: Low
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 15000
Particle Shape: Spherical
Particle Size: 3.5 µm
Endfitting Type: Waters
Pore Size: 300 Å
QC Tested: Peptide
Format: Column
Surface Area: 185
System: HPLC
Particle Technology: BEH
USP Classification: L1
Inner Diameter: 4.6 mm
Length: 150 mm
Carbon Load: 18 %
UNSPSC: 41115709
Application: Peptide
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge Peptide BEH C18 Column, 300Å, 3.5 µm, 4.6 mm X 150 mm, 1K - 15K, 1/pk The XBridge Peptide BEH C18 Column is a top of the line reversed phase column that enables you to separate peptides with confidence. The XBridge BEH300 C18 Peptide Separation Technology (PST) columns are optimized and QC tested for peptide separations. The wide-pore (300Å) trifunctionally bonded BEH particle offers you the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis. XBridge columns have been designed to contain the most chemically stable sorbent available allowing you to explore the full benefits of use in an incredibly wide pH range of 1-12. This wide range of use allows you to push the boundaries of your chromatographic capabilities with confidence. High pH stability is also associated with increased column lifetimes that will save you the cost of frequently replacing your lab equipment . The improved column reliability that the XBridge Peptide BEH C18 provides you will increase assay ruggedness. Additionally this column is designed to maximize particle efficiency to offer you unmatched peak shape and peak capacity. BEH particle technology offers you many advantages over traditional silica based particles, including the ability to control the silanol activity with great precision. By controlling the silanol activity, you reduce the unwanted silanol interactions that create peak tailing. The premier reliability that this column offers will save you the significant cost associated with the rigorous testing and validation of the final method. This column equips you with confidence that the method you develop will be repeatable for the lifetime of the assay. The XBridge Peptide BEH C18, 130Å and 300Å packing materials were designed to provide excellent peak shape, high efficiency, and excellent stability. The XBridge Peptide BEH C18 Column is rigorously tested and its materials are produced in a cGMP, ISO 9002 certified plant using an ultra pure reagent. Test results are held to narrow specification ranges to ensure excellent reproducible performance. Every column is tested and a Performance Test Chromatogram, along with a Certification of Acceptance, are provided with each column. The XBridge Peptide BEH C18 Column allows you to perform separations with 6000 PSI and a molecular range of 1,000 to 15,000. It has a designated carbon load of 18% and is intended for use with low silanol activity. This column is intended for use with spherical 3.5 micro meter particles.

FAQ
How do I equilibrate my XBridge Peptide BEH C18 Column? XBridge Peptide BEH C18 Columns are shipped in 100% acetonitrile. It is important to ensure mobile phase compatibility before changing to a different mobile phase system. Equilibrate the column with a minimum of 10 column volumes of the mobile phase to be used. To avoid precipitating out mobile phase buffers on your column or in your system, flush the column with five column volumes of a water/organic solvent mixture, using the same or lower solvent content as in the desired buffered mobile phase.