Waters, 186003952, XBridge Oligonucleotide BEH C18 Column, 130Å, 2.5 µm, 2.1 mm X 50 mm, 1/pk

Waters Oligonucleotide Separation Technology (OST) columns are based on the second generation of hybrid-silica BEH™ Technology particles functionalized with C18. The separation of detritylated synthetic oligonucleotide samples is based on the well-established method of ion-pair, reversed-phase chromatography. The availability of 1.7µm UPLC® technology or 2.5µm HPLC particles in various column dimensions provides flexibility to meet various laboratory-scale isolation or analysis needs and delivers exceptional sample resolution and superior column life. In addition, Waters manufacturing and quality control testing procedures help ensure consistent batch-to-batch and column-to-column performance regardless of application demands.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 12 pH
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 2.5 µm
Endfitting Type: Waters
Pore Size: 130 Å
QC Tested: Oligonucleotide
Format: Column
Surface Area: 185
System: HPLC
Particle Technology: BEH
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 50 mm
Carbon Load: 18 %
UNSPSC: 41115709
Application: Oligonucleotide, Gene Therapeutics
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge Oligonucleotide BEH C18 Column, 130Å, 2.5 µm, 2.1 mm X 50 mm, 1/pk Achieve fast and efficient lab-scale separations that result in greater throughput with the XBridge Oligonucleotide BEH C18 Column. The lab equipment enables you to increase your productivity through higher recoveries while offering a longer column lifetime. The column uses the Waters Oligonucleotide Separation Technology (OST) that uses the hybrid particle BEH Technology functionalized with C18. The separation of detritylated synthetic oligonucleotide samples is based on the well-established method of ion-pair, reversed-phase chromatography. The XBridge Oligonucleotide BEH C18 Column offers outstanding separation efficiencies equal to ion-exchange HPLC methods or PAGE-CGE. Its ability to resolve large oligonucleotide sequences is due to the enhanced resolving power obtained using sub-3 µm particles. In addition to this, you also get scalable column offerings for lab-scale isolation requirements. You also reduce lab operating costs with the XBridge Oligonucleotide BEH C18 Column as its excellent column life provides reduced cost per analysis. Get maximum component resolution and recovery of the oligonucleotide sample from non-desired failure sequences. You can develop robust separations in less time and with greater flexibility and confidence, leveraging the power of pH. The column offers a winning combination of cutting-edge bonding, end-capping, and particle synthesis to enable you to meet the most challenging chromatographic demands. To find out more about the technology used in the manufacturing of our lab equipment and to find products similar to the one listed here, visit our catalog. It also contains information on our various global offices and allows you to shop for lab equipment . You may also be interested in Reversed-Phase QC Reference Material , a significantly complex reference material mixture in that it contains compounds that are challenging to separate. Containing 7 compounds including uracil, butylparaben, naphthalene, propranolol, dipropyl phthalate, acenaphthene, and amitriptyline at pH 7, this mix should enable you to confirm the benchmark performance of your analytical HPLC or UPLC system before analysis of critical material.

FAQ
Is There a Temperature Range Restriction for the XBridge Oligonucleotide BEH C18 Column? The recommended operating temperatures for the XBridge Oligonucleotide BEH C18 Column are between 20 ˚C – 65 ˚C. This is in order to enhance selectivity, lower solvent viscosity and increase mass transfer rates. Please note that operating at elevated pH, temperature, and/or pressure may potentially result in shortened column life and decreased performance.