Waters, 186004496, ACQUITY UPLC Protein BEH C4 Column, 300 Å, 1.7 µm, 2.1 mm x 100 mm, 1/pk

The ACQUITY UPLC Protein BEH C4, 300 Å, 1.7 µm, 2.1 mm x 100 mm column is QC tested and optimized to separate proteins based on size, hydrophobicity and isoelectric point. If you compare to traditional C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery, and improve peak capacity.

Specifications
Chemistry: C4
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 2 pH
pH Range Max: 10 pH
Endcapped: No
Silanol Activity: Low
Molecular Weight Range Max: 150000
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 300 Å
QC Tested: Protein
Format: Column
System: UPLC, UHPLC
Particle Technology: BEH
USP Classification: L26
Inner Diameter: 2.1 mm
Length: 100 mm
Carbon Load: 8 %
UNSPSC: 41115709
Application: Protein
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
FAQs about ACQUITY UPLC Protein BEH C4 Column How does the ACQUITY UPLC Protein BEH C4 Column enhance UPLC applications for proteins? The ACQUITY UPLC Protein BEH C4 Column is tailored for UPLC applications, providing sharp peak shapes and fast separations for proteins and peptides, which is particularly beneficial when working with complex biological samples or when high throughput is required. What types of biomolecules are best separated with the ACQUITY UPLC Protein BEH C4 Column? The ACQUITY UPLC Protein BEH C4 Column is ideal for separating medium to large proteins, including monoclonal antibodies and membrane proteins, due to its large pore size and hydrophobic C4 surface. Can the ACQUITY UPLC Protein BEH C4 Column handle high throughput screening applications? Yes, the ACQUITY UPLC Protein BEH C4 Column is well-suited for high throughput screening applications due to its superior column strength and fast separation capabilities enabled by UPLC technology. What are the key considerations for solvent compatibility with the ACQUITY UPLC Protein BEH C4 Column? Solvent compatibility with the ACQUITY UPLC Protein BEH C4 Column includes the use of organic modifiers and acidic to neutral pH buffers to maintain the integrity of the biomolecules and the stationary phase during separations. What maintenance and storage guidelines should be followed for the ACQUITY UPLC Protein BEH C4 Column? The ACQUITY UPLC Protein BEH C4 Column should be maintained by flushing with appropriate solvents and stored in a solvent that ensures the stability of the bonded phase, such as 80% acetonitrile in water, as recommended by the manufacturer.

FAQ
How Should Samples Be Prepared For Use With ACQUITY UPLC BEH C4 Columns? Before injection into the system, samples should be free of all particles, as impurities can contribute to column contamination. It is recommended that samples are prepared in the operating mobile phase, or a mobile phase that is weaker than the mobile phase, to ensure optimal peak shape and sensitivity.
If samples are not dissolved in the mobile phase, ensure that sample, solvent, and mobile phase are miscible in order to avoid sample and buffer precipitation.
Filter your sample with 0.2 µm filters to remove particulates. Alternatively, centrifugation for 20 minutes at 12-50,000 g could be considered.