Waters, 186004741, ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1K - 150K, 1/pk

The ACQUITY UPLC BEH Glycan Separation Technology Column, also known as BEH Glycan, is optimized for use with ACQUITY UPLC systems with fluorescence detection (FLR), separating the released glycans of biopharmaceuticals as their 2-aminobenzamide (2-AB) derivatives.

Specifications
Chemistry: Amide
Separation Mode: Hydrophilic Interaction (HILIC)
Particle Substrate: Hybrid
pH Range Min: 2 pH
pH Range Max: 11 pH
Endcapped: No
Silanol Activity: Low
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 150000
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 130 Å
QC Tested: Glycan
Format: Column
Surface Area: 185
System: UPLC, UHPLC
Particle Technology: BEH
USP Classification: L68
Inner Diameter: 2.1 mm
Length: 100 mm
Carbon Load: 12 %
UNSPSC: 41115709
Application: Glycan
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1K - 150K, 1/pk The ACQUITY UPLC Glycan BEH Amide Column offers you unmatched resolution and batch to batch reproducibility for your liquid chromatography needs. The ACQUITY UPLC BEH Glycan Separation Technology Column, also known as BEH Glycan, is optimized for use with ACQUITY UPLC systems with fluorescence detection (FLR), separating the released glycans of biopharmaceuticals as their 2-aminobenzamide (2-AB) derivatives. When used with a correctly configured liquid chromatography instrument, this column excels at separating neutral and charged labeled glycan species. These columns achieve incredible resolving power due to the particle size of the highly porous hybrid packing material. The ACQUITY UPLC Glycan BEH Amide Column also provides you with unparalleled retention of 2-AB, 2-AA, and Rapi-Flour-MS labeled oligosaccharides because of the hydrophobicity of the molecule. The ACQUITY UPLC Glycan BEH Amide Column is an incredibly durable and long lasting column which will spare you the expensive hassle of frequently replacing lab equipment . Ultra Performance Liquid Chromatography technology enables this column to withstand high amounts of pressure and a molecular weight range of 1,000 to 150,000. The use of Ethylene Bridged Hybrid Particle Technology helps to maximize the lifetime of these extremely hardy columns. This column is also designed to function with a pH range of 2-11 and a specified carbon load of 12%.

FAQ
How do I avoid column contamination in my labeled glycans sample preparation? It is critical to the longevity of your LC columns that your samples are free of particles before they are injected into the system. Labeled glycans are usually insoluble in high acetonitrile concentrations which are common in initial HILIC conditions. Sample diluents may contain higher aqueous content than the initial composition for small volume injections. Preparation of labeled glycans could involve one or two steps of solid-phase extraction. As a result, protein precipitate has typically been removed. If not, remove protein particles by centrifugation at greater than 10,000 rpm for more than 2 minutes.