Waters, 186009175, XBridge Protein BEH SEC Column, 200 Å, 2.5 µm, 4.6 mm X 150 mm, 1/pk

XBridge Protein BEH SEC Column, 200 Å, 2.5 µm, 4.6 mm X 150 mm Columns, containing 2.5 µm particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 10,000 to 450,000 Daltons. Each batch is specifically QC tested using Waters BEH200 SEC Protein Standard (p/n: 186006518) to help ensure consistent performance for challenging size-based protein separations. Note: This item is also available with one vial the BEH200 SEC Protein Standard at no additional cost (p/n: 176004335). Suggested Use: Gel Filtration, Size Exclusion Chromatography, Aggregate, Monomer and Fragment analysis If you are developing a new method, please consider our newer technology XBridge Premier Protein 250Å SEC and ACQUITY Premier Protein 250Å SEC Columns for superior recovery, separation, and resolution.

Specifications
Chemistry: SEC
Separation Mode: Gel Filtration (aqueous)
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 4500 psi (310 Bar)
Endcapped: No
Bonding Technology: Diol (OH)
Silanol Activity: Low
Molecular Weight Range Min: 10000
Molecular Weight Range Max: 450000
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 200 Å
QC Tested: Protein
Format: Online Column
Packing Solvent: 20% methanol in water
Surface Area: 220
System: UHPLC, UPLC, HPLC
Particle Technology: BEH
Technique: LC
USP Classification: L20, L33, L59
Inner Diameter: 4.6 mm
Length: 150 mm
Carbon Load: 12 %
eCord: No
UNSPSC: 41115711
Application: Protein
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge Protein BEH SEC Column, 200 Å, 2.5 µm, 4.6 mm X 150 mm, 1/pk Invest in the XBridge Protein BEH SEC Column so you can perform analyses and make sure your analysis is producing the best results. The Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating utilized in our popular range of HPLC-based SEC columns provide the foundation for the 2.5 µm particle size, 200 Å pore size XBridge Protein BEH SEC columns. These laboratory tools work best when separating proteins with a size between 10,000 and 450,000 Daltons. You can benefit from the easy migration approach to UltraPerformance LC technology by utilizing the XBridge Protein BEH SEC Column. Due to its unrivaled column longevity and versatility to operate over an unmatched range of mobile phase pH and temperatures, the column is a crucial piece of lab equipment . The XBridge Protein BEH SEC Column uses packing materials that are produced in a cGMP, ISO 9001-certified facility using ultra-pure reagents and were created expressly for use with the ACQUITY UPLC System. To ensure repeatable performance, each batch of XBridge Protein BEH SEC Column packing material is chromatographically tested with acidic, basic, and neutral analytes, and the results are held to limited specification ranges. On the eCordTM Intelligent Chip, a Performance Chromatogram and Certificate of Batch Analysis are supplied after each column has undergone an individual test. Use the XBridge Protein BEH SEC Guard Column, 200Å, 2.5 µm, 4.6 mm X 30 mm, 1/pk in conjunction with the XBridge Protein column in order to prevent any chemical or particulate contaminants from entering the mobile phase and interfering with the analysis. The guard column is made using the same packing materials that are present in the column, making the two lab equipment well suited to work together. It also helps in delivering more accurate results and ensures that the column lasts a long time.

FAQ
Is There A Minimum And Maximum pH Limit For The XBridge Protein BEH SEC Column? The XBridge Protein BEH SEC Column listed here can work well within the 2.5-8 pH range. What Are The Temperature Limits Of The XBridge Protein BEH SEC Column? The Waters XBridge Protein BEH SEC Column listed here is able to work with a maximum of 60 C.