Waters, 186009489, ACQUITY Premier Peptide CSH C18 Column, 130Å, 1.7 µm, 2.1 x 150 mm, 1/pk

The first choice for peptide separations, Waters ACQUITY Premier Peptide CSH C18 Column, 130Å, 1.7 µm, 2.1 x 150 mm particles contain a low and carefully-defined concentration of positive charges that yield comparatively excellent peak shape for peptides using either FA- or TFA-containing mobile phases. Quality controlled with cytochrome c digest standards (p/n: 186006371) for the analysis of peptides. The fact that the performance of an ACQUITY Premier Peptide CSH C18 Column, 130Å, 1.7 µm, 2.1 x 150 mm Column exhibits little dependence on strong ion pairing agents makes it ideal for LC or LC-MS applications. 2.1mm ID columns are recommended for use with low dispersion (UPLC or UHPLC) systems, especially for LC-MS methods. This column is part of the MaxPeak Premier Class of Columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes. Suggested Use: Peptide Separations, Peptide Analysis, Peptide Mapping, Synthetic Peptides

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 11 pH
Temperature Limits: 80 C
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 130 Å
QC Tested: Peptide
Format: Premier Column
Surface Area: 185
System: UPLC, UHPLC
Particle Technology: CSH
Technique: LC
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 150 mm
Carbon Load: 15 %
eCord: Yes
UNSPSC: 41115711
Application: Peptide
Brand: ACQUITY
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY Premier Peptide CSH C18 Column, 130Å, 1.7 µm, 2.1 x 150 mm, 1/pk As part of the Waters MaxPeak Premier Class of Columns, the ACQUITY Premier Peptide CSH C18 Column will give you the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes. The column is quality controlled and tested with peptide standards for the analysis of peptides. Additionally, the 1.7 µm particles contain a low and carefully defined concentration of positive charges that yield comparatively excellent peak shape for peptides using either FA- or TFA-containing mobile phases. The lab equipment is also ideally suited for LC and LC-MS applications as it shows little dependence on strong ion-pairing agents. Use the ACQUITY Premier Peptide CSH C18 Column in order to leverage alternate selectivity that is suitable for a broad range of compounds. The Charged Surface Hybrid (CSH) particle platform that is used in the analytical column enables it to deliver exceptional peak shape and increased loading capacity. This is especially useful for basic compounds under low-pH, weak ionic strength mobile-phase conditions. The use of the ACQUITY Premier Peptide CSH C18 Column makes it possible for you to benefit from a high standard of necessary performance attributes such as superior peak shape for basic compounds, low column bleed, and efficiency. The lab equipment has been designed for the global marketplace. This means that even if your laboratories are spread across different parts of the world, using the ACQUITY Premier Peptide CSH C18 Column, you can develop robust and easily transferable methods that can be reproduced easily. Using the ACQUITY Premier Peptide CSH C18 Column, you can get the outstanding results offered by the use of TFA without its disadvantages. This is made possible by the use of the CSH technology, which provides TFA (Trifluoroacetic acid) peak shape performance without actually using TFA. In contrast, the column uses a more MS-friendly mobile phase such as formic acid and provides faster and more straightforward method development as formic acid does not exhibit the lingering chromatographic effects of an ion-pairing agent. To cater to your different analytical needs, shop for lab equipment directly from us. We recommend that you also explore the Cytochrome C Digestion Standard .

FAQ
Why Is TFA Used In HPLC? TFA is widely employed as a mobile phase additive in the HPLC separation of biological molecules, such as proteins and peptides. This is due to its ability to act as an ion-pairing reagent. It also equilibrates quickly so that it can be used with gradient elution.