Waters, 186010778, dsDNA 50 to 1350 Ladder

The dsDNA 50 to 1350 Ladder is a lyophilized mixture of a proprietary plasmid digest that is comprised of 17 double stranded DNA species, including fragments ranging from 50 to 1,350 base pairs (bp) long. By their higher abundances, the 1350, 500 and 200 bp bands can be used as reference point. The dsDNA 50 to 1350 Ladder is to be used as a performance check and potential system suitability standard for LC-UV analyses, as performed with SEC and IEX separations.

Specifications
Separation Mode: GPC/GFC/SEC
Detection Method: UV
Formulation: Solid
Storage Condition: -20C +/- 10C (Freezer)
System Type: LC
Instrument: UPLC, UHPLC, HPLC
Shelf Life: 24 Months
Shelf life upon reconstitution: 1 Week
Shipping Condition: Ambient
Tolarence: +/- 20%
Type: Resolution Check
UNSPSC: 41116107
Application: Oligonucleotide, Cell & Gene Therapy
Brand: Waters
Product Type: Standards
Units per Package: 1 pk

Additional Information
dsDNA 50 to 1350 Ladder, 1/pk The dsDNA 50 to 1350 Ladder is a lyophilized mix of 17 proprietary double-stranded DNA fragments, spanning a size range from 50 to 1350 base pairs (bp). Featuring high-abundance bands at 1350, 500, and 200 bp, it serves as an effective reference tool for LC-UV analysis in both SEC and IEX workflows. These distinct bands aid in reliable size calibration and system performance validation across analytical assays. This ladder is intended for use as a resolution and system suitability check for oligonucleotide, gene therapy, and cell therapy applications. With a solid formulation and long shelf life of 24 months (1 week post-reconstitution), it is designed to perform under ambient shipping and frozen storage conditions (-20°C). It is compatible with LC systems, including UPLC, UHPLC, and HPLC instruments, and integrates seamlessly into GMP-regulated workflows thanks to Waters' ISO-certified manufacturing standards. Need high-quality lab gear to support your bioanalytical workflows? Shop lab equipment to find the instruments and accessories you need for reproducible, reliable results. FAQs What is the dsDNA 50 to 1350 Ladder used for? This ladder is used as a resolution and system suitability standard for LC-UV analysis, particularly with SEC and IEX separations. What sizes of DNA fragments does the ladder contain? It includes 17 DNA fragments ranging from 50 to 1350 base pairs, with prominent bands at 1350 bp, 500 bp, and 200 bp for easier visualization and reference. How should the product be stored? Store at -20°C ± 10°C in a freezer. After reconstitution, the ladder has a shelf life of 1 week. What types of instruments are compatible with this ladder? It can be used with a variety of LC platforms including UPLC, UHPLC, and HPLC systems. Is this product suitable for regulated environments? Yes, it's produced in an ISO 9000-registered facility following FDA cGMP guidelines, making it suitable for regulated bioanalytical environments.