Thermo Fisher Proteins & Peptides: Reagents, Kits & Standards

Welcome! This curated collection brings together high-performing Thermo Fisher Scientific™ reagents and tools for protein and peptide workflows—spanning extraction, quantitation, LC-MS prep, purification, labeling, detection, controls, and peptide synthesis. As an authorized distributor, Iright can supply a wide range of Thermo Fisher products with flexible quotes and fast delivery to fit your lab’s needs.

Extraction & Stabilization (Cell Lysis, Inhibitors & Buffers)

Effective proteomics starts with clean, intact protein extracts. Here you’ll find lysis buffers for hard-to-lyse samples and inhibitor cocktails that preserve phosphorylation and prevent proteolysis, so downstream quantitation and MS stay reproducible. Choose EDTA-free options when metal-dependent steps (e.g., IMAC) are ahead in your workflow.

Popular picks (examples):

• RIPA Lysis & Extraction Buffer, 100 mL — 89900.
  
• Halt™ Protease & Phosphatase Inhibitor Cocktail (100X), 1 mL — 78440; 24×100 µL — 78442.
  
• Halt™ Protease Inhibitor Cocktail (100X), EDTA-free, 1 mL — 87785; standard, 1 mL — 87786.
  
• Bond-Breaker™ TCEP Solution, Neutral pH, 5 mL — 77720.

Protein Quantitation (Assay Kits & Standards)

Accurate protein concentration underpins consistent digestion, equal loading, and trustworthy quantitation. Thermo Scientific™ BCA, Micro BCA, Coomassie/Bradford, and Rapid Gold chemistries cover broad dynamic ranges and diverse sample chemistries—including detergent-rich lysates.

Go-to solutions:

• Pierce™ BCA Protein Assay Kit — 23225.
  
• Micro BCA Protein Assay Kit — 23235.
  
• Coomassie Plus (Bradford) Protein Assay Kit — 23236.
  
• Rapid Gold BCA (standard format A53227 or Dilution-Free A55860).
• BSA standards, 2 mg/mL ampules — 23209.

Digestion & Sample Prep for LC-MS

Enzyme choice and clean-up define coverage and sensitivity. Use MS-grade proteases for reproducible cleavage, then desalt and remove surfactants with spin columns to protect your LC-MS and boost IDs. The products below are proven, Orbitrap-friendly workhorses.

Representative SKUs

Purification & Affinity (His-Tag & Antibody Tools)

Affinity capture improves purity, yield, and confidence in downstream assays. Nickel or cobalt IMAC resins enrich His-tag proteins, while Protein A/G magnetic beads streamline IPs and antibody purification on magnets or robots.

Representative SKUs

Labeling & Crosslinking (Amine-Reactive Dyes & Crosslinkers)

From antibody biotinylation to distance-constrained crosslinking MS, high-purity reagents ensure specific, stable modifications. Select PEGylated biotin for solubility, NHS esters for primary amines, and cleavable crosslinkers when MS identification demands it.

Representative SKUs

Detection for WB/ELISA (Substrates, Membranes & Secondaries)

Reliable detection tightens your limit of detection and dynamic range. Choose ECL substrates matched to abundance, PVDF for strong protein binding, and validated HRP secondaries for clean blots and ELISAs.

Representative SKUs

Standards & Controls (MW Markers & System Suitability)

Standards help you compare across runs and instruments. Pair BSA standards for assay calibration with molecular-weight ladders for gel validation, and add PRTC peptides to monitor LC-MS performance over time.

• PageRuler™ Plus Prestained Protein Ladder (10–250 kDa) — 26619.
  
• Pierce™ Unstained Protein MW Marker — 26610.
  
• Pierce™ BSA Standards, 2 mg/mL — 23209.
  
• Pierce™ PRTC Peptide Mixture — 88321.

Peptide Synthesis Essentials (Reagents, Resins & Building Blocks)

For solid-phase peptide synthesis (SPPS), reliable coupling reagents and high-purity Fmoc-amino acids maintain sequence fidelity and yield. Thermo Scientific™ Chemicals (Acros/Alfa Aesar) provides widely used couplers and resins compatible with standard Fmoc strategies.

Representative SKUs

Storage & Handling (Quick Guidance)

Proper storage preserves activity and performance. Many inhibitors are shipped cold and stored at 4 °C; ECL substrates and enzymes often require refrigeration; fluorescent dyes and NHS esters are typically kept desiccated at −20 °C, protected from light. Always follow each product’s datasheet for exact conditions.

Application Matrix (Choose-by-Need)

FAQs

Q1. Are Pierce™, Invitrogen™, and Thermo Scientific™ products compatible across workflows?
Yes—these Thermo Fisher brands are designed to interoperate across extraction, quantitation, LC-MS, immunoassays, and imaging. Match chemistries (e.g., EDTA-free with IMAC; MS-grade reagents for LC-MS) for best results.

Q2. Which ECL should I choose for Western blot?
Use West Pico PLUS for most targets; switch to West Femto for very low-abundance proteins. Pair with PVDF membranes and optimized blocking to minimize background.

Q3. When should I pick cobalt over nickel for His-tag purification?
Cobalt generally gives higher purity with slightly lower capacity—use when selectivity matters most or contaminants persist after Ni-NTA.

Q4. Do I need detergent removal before LC-MS?
Yes, typical ionic and nonionic detergents suppress ionization. Use Detergent Removal Spin Columns or C18 cleanup prior to injection.

Q5. What’s the advantage of DSSO vs. DSS/BS³?
DSSO is MS-cleavable, simplifying identification of crosslinked peptides; DSS/BS³ are non-cleavable workhorses for structural mapping and stabilization.

Contact for a Quote

Need a tailored quote, bulk packaging, or cross-workflow guidance? Contact Iright to source any Thermo Fisher™ Proteins & Peptides product above—including alternatives, sizes, and regional lead times. We’ll help you select, bundle, and deliver exactly what your experiments require.