BD, 556375, BD Pharmingen™ Purified NA/LE Mouse Anti-Human CD178

Alternative Name: Fas Ligand, CD95 Ligand
Reactivity: Human (QC Testing)
Isotype: Mouse IgG2a
Immunogen: L51788Y Mouse T Lymphoma Cells Expressing Recombinant Human FasL
Application: Immunoprecipitation (Routinely Tested), Flow cytometry (Tested During Development), ELISA, Neutralization (Reported), Western blot (Not Recommended)
Target Molecular Weight: 40, 26 kDa
Concentration: 1.0 mg/ml
RRID: AB_396396
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures IP : Applications include immunoprecipitation (1-2 µg/one million cells). Functional Assay : NOK-2 has also been shown to neutralize the cytotoxic activity of FasL. Neutralization of FasL activity inhibits Fas-mediating  killing. The NA/LE format of NOK-2 (Cat. No. 556375) should be used for all functional assays. NOK-2 and a related human FasL clone, NOK-1 [Cat. No. 556371 (NA/LE)] may give different profiles in neutralization assays. It is thought that NOK-1 and NOK-2 likely recognize different FasL epitopes. Flow Cytometry : Another clone,  NOK-1, (556372, 556373)  are  recommended for flow cytometry. NOK-2 appears weaker for flow cytometry than NOK-1. Western Blot : Neither NOK-1 nor NOK-2 are suggested for western blot analysis. Another human FasL clone, G247-4 (Cat. No. 556387) is suggested for the western blot analysis.