BD, 560564, BD Pharmingen™ FITC Rat Anti-Mouse IL-12 (p40/p70)

Reactivity: Mouse (QC Testing)
Isotype: Rat IgG1
Immunogen: CHO-expressed recombinant mouse IL-12 p70 protein
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_1645243
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Recommended Assay Procedures: Recommended Assay Procedures Flow cytometry: The FITC Rat Anti-Mouse IL-12 (p40/p70) antibody can be used for the enumeration of IL-12 producing cells within mixed cell populations. A useful control investigators may consider using for demonstrating specificity of staining is to pre-block with one of the following reagents:  (1) recombinant mouse IL-12 (p40) (Cat. No. 554594) or with (2) unlabeled C15.6 antibody (Cat. No. 554477), prior to staining.  Intracellular staining techniques and the use of blocking controls are described by C. Prussin and D. Metcalfe.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.