BD, 565173, BD Horizon™ APC-R700 Mouse Anti-Human CD196 (CCR6)

Alternative Name: BN-1; C-C CKR-6; C-C chemokine receptor type 6; CC-CKR-6; CCR-6
Reactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
Isotype: Mouse IgG1, κ
Immunogen: Human CD196/CCR6 Peptide
Application: Flow cytometry (Routinely Tested)
Vol. Per Test: 5 µl
Workshop Number: IX 48
RRID: AB_2739092
Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, glycerol and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon APC-R700 under optimum conditions, and unconjugated antibody and free BD Horizon APC-R700 were removed.
Recommended Assay Procedures: Recommended Assay Procedures For the best staining of CD196 with the 11A9 antibody, BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) is strongly recommended.
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Source of all serum proteins is from USDA inspected abattoirs located in the United States. An isotype control should be used at the same concentration as the antibody of interest. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.