Bio-Rad, 1725140, iTaq Universal Probes One-Step Kit, 100 x 20 µl rxns, 1 ml

100 x 20 µl reactions, 1 ml (1 x 1 ml) 2x one-step reaction mix, containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript reverse transcriptase; and nuclease-free water
Overview
Use our combined iScript RNase H + reverse transcriptase and antibody hot-start iTaq DNA polymerase probe kit formulation to complete your real-time reactions in one step — PCR efficiency is improved over a wide dynamic range and under various conditions.
Key Features and Benefits
• Sensitive and reproducible singleplex or multiplex reactions directly from RNA
• Advanced formulation for amplifying difficult target sequences and challenging samples
• Tested with ethanol for inhibitor tolerance and compatible with up to 4 µl crude lysate
• Works on any real-time PCR system
Kit Components
• Reverse transcriptase — 50 µl
• Reaction mix — 1 ml
Instrument Compatibility
iTaq Universal Probes One-Step RT-qPCR Kit is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
• StepOne/StepOnePlus
• 7500, ViiA 7
• 7000, 7300, 7700, 7900HT, QuantStudio Systems
• Mx3000P, 3005P, 4000
• Mastercycler ep realplex 2, 4
• Corbett Rotor-Gene 3000, 6000, Q
• LightCycler 480, 96
• LightCycler 1.0, 1.5, 2.0*
• LightScanner HR-1
• LightScanner 32*
* BSA must be added according to instrument specifications
Applications and Uses of the iTaq Universal Probes One-Step Kit
• qPCR/real-time PCR
• Gene expression analysis
• Multiplexing
• Absolute quantification
• Mutation detection
• Pathogen detection
• Viral detection (load)
• Genetically modified organisms (GMO) characterization
• Genetic profiling
Packaging Options
The iTaq Universal Probes One-Step Kit is also available in a format of 500 reactions ( 1725141 ) and 5,000 reactions ( 12013250 ).
Related Products
Bio-Rad has also developed one-step kits for SYBR ® Green specific applications .