Merck, I5260-1ML, Anti-Human IgG (Fab specific) antibody produced in goat

Anti-Human IgG (Fab specific) antiserum is produced in goat using purified human IgG Fab fragment as the immunogen. Affinity isolated antibody is obtained from goat anti-human IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. IgG is a larger molecule with a molecular weight of 150 kilo daltons (kDa). IgG has two light chains (L) and two heavy chains (H). The light chain can be either lambda (λ) or kappa (κ) chain. Functionally, immunoglobulins have constant region and variable region. Light chain has one variable region in the N-terminal VL and followed by one constant domain CL. The light chain is approximately about 25 kDa. The heavy chain has one variable region in the N-terminal and followed by three constant domains. In between the first (C1H) and second constant (C2H) domain in the spacer hinge region which connects both the heavy chains. Each heavy chain is about 55 kDa.