Qiagen, 37582, Qproteome Nuclear Protein Kit

For 12 nuclear protein preparations: Buffers, Reagents, Protease Inhibitor Solution, Benzonase

Features

- Nuclear preparations free of cytosolic proteins
- Greatly reduced sample complexity
- Highly reproducible fractionation
- Includes protocols for tissue proteomics

Product Details

The Qproteome Nuclear Protein Kit is designed for specific enrichment of nuclear proteins from cultured mammalian cells.

Principle

The Qproteome Nuclear Protein Kit is designed for specific enrichment of nuclear proteins from cultured mammalian cells. Lysis and centrifugation are used to separate the cytosolic fraction (supernatant) from the cell nuclei (pellet). A high-salt buffer allows dissociation of nuclear binding proteins (such as transcription factors) and their removal by diffusion from the nuclei.

Applications

The Qproteome Nuclear Protein Kit delivers a nucleic acid binding protein fraction suitable for a wide range of activity assays.