Qiagen, 37612, Qproteome Mitochondria Isolation Kit

Buffers and reagents for 12 high-purity mitochondrial preparations

Features

- High-purity, intact mitochondria
- Highly reproducible using a standardized procedure
- Convenient, fast, and easy-to-use kit format

Product Details

The Qproteome Mitochondria Isolation Kit provides fully active mitochondrial preparations of very high purity in a fast, reproducible, and standardized procedure that uses a standard bench-top centrifuge.

Performance

In contrast to a differential centrifugation method from the literature, analysis of cell-compartment marker proteins shows that the Qproteome Mitochondria Isolation Kit provides mitochondrial fractions virtually free of cross-contamination (see figure "Mitochondrial fractions virtually free of cross-contamination" ). The virtual absence of cytosolic contaminants greatly facilitates analysis of low-abundance species as shown in 2D-PAGE analyses and the gentle procedure provides a highly enriched fraction containing intact mitochondria (see figure " High purity mitochondria with intact mitochondrial membranes "). Integrity can also be demonstrated by measuring uptake of a fluorescent dye (see figure "More intact mitochondria per milligram protein" ).

Principle

One of the major problems facing researchers working with mitochondria is efficiently separating mitochondrial and cytosolic fractions. Traditional preparation methods are tedious, time-consuming, and require access to an ultracentrifuge. The Qproteome Mitochondria Isolation Kit provides fully active mitochondrial preparations of very high purity in a fast, reproducible, and standardized procedure that uses a standard bench-top centrifuge.

Applications

Mitochondria are the subject of intense study in a number of fields, including apoptosis, proteomics, biomedical research, and drug discovery. The Qproteome Mitochondria Isolation Kit delivers high-purity mitochondria whose proteins retain their native conformation and full biological activity — making them highly suited for apoptosis studies, 2D-PAGE analysis, and other biochemical assays.