Qiagen, WB120028, QIAcard FTA CloneSaver (5), 96 sample areas per card

5 cards

Features

- Plasmid protection from nucleases, oxidation, UV damage, and microbial and fungal attack
- Direct electroporation from punched disk or
- Electroporation and heat-shock transformation from card eluate
- Room temperature shipment of plasmid and BAC DNA clones

Product Details

The QIAcard FTA CloneSaver format is designed for the long-term, room-temperature storage of plasmid and BAC DNA clones. The unique FTA technology enables years of ambient clone archiving that condenses the functions of −80°C freezers and purification kits down to the size of an index card. FTA technology is able to lyse cells on contact and sequester the DNA regardless of glycerol content or growth media. Once separate from the lysed cell and bound to the CloneSaver fibers, the DNA is ready for room temperature storage in a lab drawer, card catalog or lab notebook. The QIAcard FTA CloneSaver format protects nucleic acids from nucleases, oxidation, UV damage, and microbial and fungal attack.

Procedure

Samples that can be applied to QIAcard FTA CloneSaver include overnight cultures of bacteria, suspended colonies, glycerol stocks and purified plasmid DNA. The sample area is formatted in a 96-well configuration. Samples applied to the card as directed will not contaminate other samples in adjacent circles. The pink-colored QIAcard FTA CloneSaver changes to white upon application of liquid samples allowing easy identification of sample location. Samples stored on QIAcard FTA CloneSaver and enclosed in a multibarrier pouch may be shipped to collaborators through the post.

Applications

DNA stored on the QIAcard FTA CloneSaver format is suitable for many common downstream applications including PCR and transformation (electroporation and heat-shock), rolling circle amplification and sequencing applications. Once the plasmid/BAC DNA is clean of any cell debris that may have been present and the disk is clean of any residual FTA chemical, the clone of interest is ready to be analyzed via PCR.