Thermo Fisher, 16159, Pierce™ Gaussia Luciferase Flash Assay Kit Thermo Scientific™

Pierce™ Gaussia Luciferase Flash Assay Kit Thermo Scientific™ The Thermo Scientific PierceGaussiaLuciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
Features of theGaussiaLuciferase Flash Assay Kit:
•Sensitive—1000 times greater sensitivity allows utilization of smaller numbers of cells
•Cost effective—highest sensitivity assays result in decreased reagent consumption
•Secreted luciferase—allows real-time assays and kinetic studies without destroying cells
•Time-saving—assays using secreted luciferases require minimal sample handling
•Automation-friendly—amenable to high throughput screens
•Convenient—contains a universal cell lysis buffer and optimized flash assay reagent
•Safe—allows one to perform non-radioactive assaysThis Flash Assay Kit contains reagents for measuring the activity ofGaussialuciferase in mammalian cell culture media and lysates. When used with Thermo ScientificGaussiaLuc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity.
Gaussialuciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. The signal produced byGaussiais considerably greater than signal from either firefly orRenillaluciferases assayed under similar conditions.
Includes:Cell lysis buffer, reaction buffer and substrateRequires:Gaussialuciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.
Applications:
• Promoter studies for analyzingcis- regulatory elements andtrans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Secretory pathway/protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studiesGaussialuciferase is a 20kDa protein from the marine copepod,Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount ofGaussialuciferase protein produced and used to determine the activity of the promoter drivingGaussiaexpression. The signal produced byGaussialuciferase shows strong flash kinetics and is considerably greater than flash signals from either firefly orRenillaluciferases assayed under similar conditions. The assay kit is optimized for use with ourGaussiaLuc Vectors but is fully compatible with otherGaussialuciferases and derivatives that use coelenterazine as a substrate. (For glow assays, use ourGaussia-Dura Luc Vectors.)More Product Data
•Highly sensitive multiplex luciferase reporter assays
•Luciferase assays in hard-to-transfect Jurkat cells
•Monitoring neuronal differentiation using multiplexed luciferase reporters
•Activation of the antioxidant response pathway by pesticide chemicals
•Versatile luciferases: microplate luminometers and flash luciferase assaysAssay: Reporter Enzyme, Luciferase Reporter Assay
Compatible Cells: Mammalian Cells
Detection Method: Bioluminescence
For Use With (Equipment): Luminometer (Microplate)
Format: 384-well plate, 96-well plate
Label Type: Enzyme Labeled
Product Line: Pierce™
Quantity: 1000 Reaction Kit
Substrate: Coelenterazine
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Sufficient For: 1000 Luciferase Assays
Target: Luciferase, Gaussia Luciferase
Technique: Enhanced Chemiluminescence
Type: Assay Kit
Unit Size: Each