Thermo Fisher, 16174, Pierce™ Firefly Luciferase Flash Assay Kit

100 Reaction Kit The Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.
Features of the Firefly Luciferase Flash Assay Kit:
• Highly sensitive detection of firefly luciferase activity
• Use withPierce Firefly Luciferase Signal Enhancerfor best results (maximum signal)
• Working solution stable for up to 2 months at -20°C
• Assay reagents compatible with other firefly luciferases
• Amenable to automation
• Contains a universal cell lysis buffer and optimized flash assay reagent
• Safe non-radioactive assayThermo Scientific Pierce Firefly Luciferase Flash Assay Kit contains reagents to measure the activity of firefly luciferase in mammalian whole cell lysates. Containing a convenient lysis buffer, assay buffer and firefly substrate, this kit provides a complete reagent system for the intracellular detection of promoter or pathway activity. Light output resulting from the luciferase reaction is captured using a luminometer and can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression. These reagents are optimized to work with Thermo Scientific Firefly Luc Plasmids; however, the Flash Assay Kit may be used to detect the activity of other firefly or ATP-dependent luciferases utilizing D-luciferin as a substrate.
Includes:Cell lysis buffer, reaction buffer and substrate; purchaseLuciferase Signal Enhancerseparately.
Requires:
• Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.
• For best results (maximum signal), use withPierce Firefly Luciferase Signal EnhancerApplications:
• Promoter studies for analyzingcis- regulatory elements andtrans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Secretory pathway/protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studiesFirefly luciferase is a 60kDa protein produced in nature by several species of the Lampyridae family of beetles which includes the generaPhotinusandLuciola. Bioluminescent signal from firefly luciferase originates from the oxidation of D-luciferin. Light output captured using a luminometer can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression.
More Product Data
•Monitoring neuronal differentiation using multiplexed luciferase reporters
•Activation of the antioxidant response pathway by pesticide chemicals
•Versatile luciferases: microplate luminometers and flash luciferase assaysAssay: Reporter Enzyme, Luciferase Reporter Assay
Compatible Cells: Mammalian Cells
Detection Method: Bioluminescence
For Use With (Equipment): Luminometer (Microplate)
Format: 384-well plate, 96-well plate
Label Type: Enzyme Labeled
Product Line: Pierce™
Quantity: 100 Reaction Kit
Substrate: D-Luciferin
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Target: Luciferase, Firefly Luciferase
Technique: Enhanced Chemiluminescence
Type: Assay Kit
Unit Size: Each