Thermo Fisher, 18011015, Bacterial Alkaline Phosphatase

Thermo Fisher, 18011015, Bacterial Alkaline Phosphatase Bacterial Alkaline Phosphatase (BAP) removes 3´ and 5´ phosphates from DNA and RNA. BAP is active at 65°C for at least 1 h and is inactivated by phenol extraction.
Applications:Dephosphorylation of 5´-phosphorylated termini of vector DNA to prevent self-ligation. Dephosphorylation of 5´ termini of nucleic acids prior to forward reaction with kinase.
Source:Purified fromE. coliC90.
Performance and Quality Testing:Endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; dephosphorylationefficiency determined.
Unit Definition:One unit hydrolyzes 1 nmol of ATP in 30 min. at 37°C.
Unit Reaction Conditions:10 mM Tris-HCl (pH 8.
0), 1.
5 mM [γ-32P]ATP, and enzyme in 50 μl for 30 min. at 37°C.
Product Type: Alkaline Phosphatase
Quantity: 2500 U
Shipping Condition: Wet Ice
Enzyme: BAP
Unit Size: Each