Thermo Fisher, 18288019, MAX Efficiency™ DH5αF'IQ Competent Cells

MAX Efficiency™ DH5αF'IQ Competent Cells MAX Efficiency DH5αF´IQ Competent Cells are high transformation efficiency chemically competent cells prepared by a patented modification of the procedure of Hanahan. This strain is a derivative of the DH5α strain, sharing the same benefits. The DH5αF´IQ strain contains an F´ episome and is a host for M13mp cloning vectors. It also supports cloning plasmids with an f1-like origin of replication (phagemids) or vector that uses expression from thelacpromoter. DH5αF´IQ cells can yield >1 x 108plaque forming units/μg M13mp19 RF with non-saturating amounts (5 pg) of DNA.
MAX Efficiency DH5αF´IQ Competent Cells have thelacZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. They also carry therecA1 mutation which helps to reduce the rate of recombination while propagating plasmid DNA and theendA1 mutation that increases plasmid DNA yield and quantity.
MAX Efficiency DH5αF´IQ Competent Cells offer:
• >3 x 108transformants/μg efficiency
• >1 × 108plaque forming units/μg M13mp19 RF
• Stable F´ episome that eliminates need for growth on minimal media
•lacZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal
• Plasmid DNA maintenance with minimum recombination events duerecA1 mutation
•endA1 mutation that enables increased plasmid yield and quantityNote:DH5αF'IQ lawn cells are provided to allow for plaque formation and a vial of monomer M13mp18 RF DNA are supplied as a control.
GenotypeF–φ80lacZΔM15 Δ(lacZYA-argF)U169recA1endA1hsdR17 (rK–, mK+)phoAsupE44 λ–thi–1gyrA96relA1/F′ [proAB+lacIqZΔM15zzf::Tn5(KmR)]Find the strain and format that fits your needsTheDH5α strain and its derivativesare available with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats.
DH5α-T1Rcompetent cells are available inMultiShot formatfor high throughput applications.
Explorebacterial growthmedia formats.
Antibiotic Resistance Bacterial: Yes (Kanamycin)
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: No
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: Yes
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Preparing Unmethylated DNA: No
Product Line: MAX Efficiency™
Product Type: Chemically Competent Cells
Quantity: 5 x 200 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency Level: Medium Efficiency (1 x 108to 1 x 109cfu/μg)
Format: Tube
Promoter: Laclq
Species: E. coli (K12)
Unit Size: Each