Thermo Fisher, 21650, Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)

Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate) Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.
Features of Sulfo-LC-SPDP:
•Reactive groups:NHS esterandpyridyldithiol
•Reactive towards: amino and sulfhydryl groups
• Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
• Water soluble, sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
• Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.
5
• Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading buffer
• Cleavable crosslinker allows separation of crosslinked products
• Sulfo-LC-SPDP crosslinker is not membrane permeable, allowing cell surface labeling
• Compare to other varieties of SPDP-type reagents, including pegylated formsSulfo-LC-SPDP is Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate, a heterobifunctional, thiol-cleavable and membrane impermeable crosslinker. It contains an amine-reactiveN-hydroxysuccinimide (NHS) esterthat will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in thepyridyl disulfidegroup that will react with sulfhydryls to form a reversible disulfide bond.
Applications:
• Reversible crosslinking of proteins
• Adding sulfhydryl groups to proteins by attachment to lysine residues
• Protein crosslinking to create antibody-enzyme and other bioconjugates via a two-step reaction
• Attaching haptens to carrier proteins for antibody production
• Preparing immunotoxin conjugatesSpecifications:We manufacture Sulfo-LC-SPDP to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of Sulfo-LC-SPDP is tested to meet the following minimum specifications.
• Identity: IR scan shows only peaks characteristic of the structure and functional groups of Sulfo-LC-SPDP crosslinker
• Solubility: ≥ 95 mg/mL in DI water
• Purity: ≥ 90% by quantitative NMR (the highest standard for crosslinker purity)Product References:Crosslinker Application Guide -- search for recent literature references for this productCell Permeability: No
Description: Sulfo-LC-SPDP
Form: Powder
Labeling Method: Chemical Labeling
Molecular Weight (g/mol): 527.
57
PEGylated: No
Product Line: Pierce™
Quantity: 50 mg
Reactive Moiety: Pyridyldisulfide, Sulfo-NHS Ester
Shipping Condition: Ambient
Solubility: Water
Spacer Arm Length: 15.
7 Å
Water Soluble: Yes
Chemical Reactivity: Amine-Sulfhydryl
Cleavable: By Thiols
Crosslinker Type: Heterobifunctional
Format: Standard
Product Type: Crosslinker
Spacer: Medium (10 to 30 Å)
Unit Size: Each