Thermo Fisher, 22301, PDPH (3-(2-pyridyldithio)propionyl hydrazide)

PDPH (3-(2-pyridyldithio)propionyl hydrazide) Thermo Scientific Pierce PDPH is a short, pyridyldithiol-and-hydrazide crosslinker for reversibly conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
Features of PDPH :
•Reactive groups:pyridyldisulfideandhydrazide
•Reactive toward: sulfhydryl groups and carbonyl (aldehyde) groups
• Short (9.
2A), sulfhydryl-to-aldehyde crosslinker with disulfide bond spacer arm (cleavable)
• Pyridyldithiol group results in attachment to sulfhydryls via disulfide bond, which can be cleaved with DTT, TCEP or other reducing agents
• Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
• Usesodium meta-periodateto oxidize glycosylation (e.
g., sialic acid) to reactive aldehyde groups
• Use withEDCto conjugate primary amine of hydrazide group to carboxyl groupsProduct References:Crosslinker Application Guide -- search for recent literature references for this productCell Permeability: Yes
Description: PDPH
Form: Powder
Labeling Method: Chemical Labeling
Molecular Weight (g/mol): 229.
32
PEGylated: No
Product Line: Pierce™
Quantity: 50 mg
Reactive Moiety: Hydrazide, Pyridyldisulfide
Shipping Condition: Ambient
Solubility: DMF, DMSO
Spacer Arm Length: 9.
2 Å
Water Soluble: No
Chemical Reactivity: Carbohydrate-Sulfhydryl
Cleavable: By Thiols
Crosslinker Type: Heterobifunctional
Format: Standard
Product Type: Crosslinker
Spacer: Short (<10 Å)
Unit Size: Each