Thermo Fisher, 22362, LC-SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxy-(6-amidocaproate))

LC-SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxy-(6-amidocaproate)) Thermo Scientific Pierce LC-SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long cyclohexane-stabilized spacer arm (16.
2 angstroms).
Features of LC-SMCC:
•Reactive groups:NHS esterandmaleimide
•Reactive toward:amino and sulfhydryl groups
• Long-chain variety ofSMCC
• High purity, crystalline reagent can be used to create high-purity maleimide-activated derivatives
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO)
• Cyclohexane bridge confers added stability to the maleimide group making LC-SMCC an ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.
1 M sodium phosphate buffer, pH 7 at 4°C.
LC-SMCC is a non-cleavable and membrane permeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.
5-7.
5 to form stable thioether bonds. The maleimide groups of LC-SMCC and are unusually stable up to pH 7.
5 because of the cyclohexane bridge in the spacer arm.
Applications:
• Ideal reagent for enzyme labeling of antibodies, both enzyme activity and antibody specificity can be preserved
• Create specific bioconjugates via one- or two-step crosslinking reactions
• Create sulfhydryl-reactive, maleimide-activated carrier proteins for coupling haptensLC-SMCC is often used for the preparation of antibody-enzyme and hapten-carrier conjugates. In this type of conjugation, the NHS ester is reacted first with the antibody, excess crosslinking reagent removed and then the sulfhydryl-containing enzyme molecule is added. This two-step reaction scheme results in formation of specific antibody-enzyme conjugates.
LC-SMCC is a reagent of choice for creating stable maleimide-activated carrier proteins that will spontaneously react with sulfhydryls. In this application, the NHS ester of LC-SMCC is reacted with lysine residues on the carrier protein, converting them to reactive maleimides. These relatively stable maleimide-activated intermediates may be lyophilized and stored for later conjugation to a hapten.
Specifications:We manufacture LC-SMCC to the highest specifications to produce the most specific bioconjugates and maleimide-activated proteins, ensure the integrity of your data and to provide you with the highest degree of crosslinking consistency. Each lot of LC-SMCC is tested to meet the following minimum specifications.
• Identity: IR scan shows only peaks characteristic of the structure and functional groups of LC-SMCC crosslinker
• Purity: ≥ 90% by NMRProduct References:Crosslinker Application Guide -- search for recent literature references for this productCell Permeability: Yes
Description: LC-SMCC
Form: Solid
Labeling Method: Chemical Labeling
Molecular Weight (g/mol): 447.
48
PEGylated: No
Product Line: Pierce™
Quantity: 50 mg
Reactive Moiety: Maleimide, NHS Ester
Shipping Condition: Ambient
Solubility: DMF, DMSO
Spacer Arm Length: 16.
2 Å
Water Soluble: No
Chemical Reactivity: Amine-Sulfhydryl
Cleavable: No
Crosslinker Type: Heterobifunctional
Format: Standard
Product Type: Crosslinker
Spacer: Medium (10 to 30 Å)
Unit Size: Each