Thermo Fisher, 23266, Pierce™ Fluorescent Protease Assay Kit

Pierce™ Fluorescent Protease Assay Kit The Thermo Scientific Pierce Fluorescent Protease Assay Kit measures total protease activity in samples, providing a means to assess the progress of protease isolation procedures or quantify protease contamination in biological samples.
This kit includes fluorescein-labeled casein as a substrate for assessing protease activity in a sample by either fluorescence resonance energy transfer (FRET) with a standard fluorometer or fluorescence polarization (FP) with capable instrumentation. FTC-Casein is native casein that has been labeled using a large molar excess of fluorescein isothiocyanate (FITC). Fluorescence properties of this heavily-labeled, intact protein substrate change dramatically upon digestion by proteases, resulting in a measurable indication of proteolysis.
Features of the Fluorescent Protease Assay Kit:
•Casein substrate—measure activity of any protease that cleaves casein into peptide fragments
•Trypsin standard—quantify protease activity relative to trypsin, a universally accepted reference
•Sensitive—1000 times more sensitive than assays that use unmodified forms of casein
•Simple and fast—test tube and microplate protocols are completed in less than one hour
•Homogenous—addition steps only; no separation, transfer or stop steps required
•Customizable—easily adapt time, temperature and pH to optimize sensitivity for proteases of interestApplications:
• Assess functional integrity and overall progress of protease purification
• Quantify protease contamination in protein samples
• Characterize the activity of unusual proteases compared to trypsin
• Evaluate buffer conditions for their affects on protease function and activityAdditional Specifications:
• Substrate: fluorescein-labeled casein (FITC-casein); available separately as Part No. 23267
• Basis of assay: change in fluorescence resonance (FRET) or fluorescence polarization (FP)
• Measurement: instrument with fluorescein excitation and emission filters (485/538nm)Assay: Protease Assay
For Use With (Application): Fluorescence Polarization, FRET
For Use With (Equipment): Microplate Reader
Product Line: Pierce™
Product Type: Protein Activity Assay
Quantity: 2000 Test Kit
Substrate: Casein
Substrate Properties: Protein-Based Substrate
Substrate Type: Protease Substrate
Sufficient For: 2000 Microplate Assays
Target: Proteases
Detection Method: Fluorescence
Immunoassay Kit Format: 96-well Plate
Label Type: Classic Dye
Unit Size: Each