Thermo Fisher, 54200, Pierce™ Protein A/G IgG Binding Buffer

Pierce™ Protein A/G IgG Binding Buffer Thermo Scientific Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads. The Protein A/G IgG Binding Buffer is a phosphate-based buffer containing EDTA, formulated for antibody purification using either Protein A or Protein G.
These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.
The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.
Shared features of all Thermo Scientific Pierce IgG Binding and Elution Buffers:
•Ready to use—carefully formulated and prefiltered 1X solutions
•Three binding buffers—optimized for Protein A, Protein G or Protein A/G
•Two elution buffer options—acidic (pH 2.
8) or high salt (near-neutral)
•Versatile—elution buffers are appropriate for antibody-antigen affinity purificationProducts available for each antibody purification method:
•Protein A IgG Binding Buffer:pH 8.
0; phosphate-based; contains glycerol and EDTA
•Protein A/G IgG Binding Buffer:pH 8.
0; phosphate-based; contains EDTA
•Protein G IgG Binding Buffer:pH 5.
0; contains 0.
02% sodium azide
•IgG Elution Buffer:pH 2.
8; amine-based
•Gentle Ag/Ab Binding Buffer:pH 8.
0; phosphate-free; contains 0.
05% sodium azide
•Gentle Ag/Ab Elution Buffer:pH 6.
6; high salt (ionic strength)Description: Pierce Protein A/G IgG Binding Buffer
Form: Liquid
Quantity: 240 mL
Reagent Type: Binding Buffer
Product Line: Pierce™
Product Type: Binding Buffer
pH: 8
Unit Size: Each