Thermo Fisher, A10270, Alexa Fluor™ 594 Azide (Alexa Fluor™ 594 Carboxamido-(6-Azidohexanyl), Triethylammonium Salt), mixed isomers

Alexa Fluor™ 594 Azide (Alexa Fluor™ 594 Carboxamido-(6-Azidohexanyl), Triethylammonium Salt), mixed isomers The red-fluorescent Alexa Fluor® 594 azide can be reacted with terminal alkynes via a copper-catalyzed click reaction. Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves a copper-catalyzed triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the components of the reaction are bioinert; and perhaps most importantly, no side reactions occur – the label and detection tags react selectively and specifically with one another. Unlike traditional chemical reactions utilizing succinimidyl esters or maleimides that target amines and sulfhydryls – functional groups that are not unique – click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background.
Chemical Reactivity: Alkyne
Detection Method: Fluorescence
Emission: 617
Excitation: 590
Format: Solid
Label or Dye: Alexa Fluor™ 594
Product Type: Azide
Quantity: 0.
5 mg
Reactive Moiety: Amine, Azide
Shipping Condition: Room Temperature
Solubility: DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
System Type: Click-iT™
Color: Red, Red
Label Type: Alexa Fluor
Product Line: Alexa Fluor™, Molecular Probes™
Unit Size: Each