Thermo Fisher, AM2141, Ambion™ T4 RNA Ligase, cloned, 5 U/μL

Ambion™ T4 RNA Ligase, cloned, 5 U/μL Ambion™ T4 RNA Ligase catalyzes the formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor. Substrates include RNA, DNA, and oligonucleotides. One tube containing 2,500 U (5 U/ μL) is provided along with 10X Reaction Buffer. The enzyme can be used for tagging the 5' ends of mRNA with oligonucleotides for mapping studies (RACE), 3'-end labeling RNA molecules, and circularizing RNA and DNA molecules. Unit Definition:One unit catalyzes the formation of 1 nmol of [5'-32P]-rA12-18into a phosphatase-resistant form in 30 min at 37°C. Concentration: 5 U/μL
Enzyme: Ligase
Fidelity (vs. Taq): 10 X
Form: Solution
No. of Reactions: 250
Polymerase: SP6 RNA Polymerase
Product Line: Ambion™
Product Type: T4 Rna Ligase
Quantity: 2,500 units, 5 μL
Shipping Condition: Dry Ice
Source: RNA
Format: Tube
Unit Size: Each