Thermo Fisher, G22183, Guanosine 5'-O-(3-Thiotriphosphate), BODIPY™ FL Thioester, Sodium Salt (BODIPY™ FL GTP-γ-S, Thioester)

Guanosine 5'-O-(3-Thiotriphosphate), BODIPY™ FL Thioester, Sodium Salt (BODIPY™ FL GTP-γ-S, Thioester) BODIPY FL GTP-γ-Sbinds to GTP-binding proteins. To create this fluorescent nucleotide, the BODIPY FL fluorophore was linked through the γ-thiol of GTP-γ-S. The fluorescence of BODIPY FL GTP-γ-Sis quenched ∼90% relative to that of the free dye, but may be recovered on binding to G-proteins. In addition to its potential use for binding studies, the BODIPY FL GTP-γ-Sthioether is an important new substrate for Fhit, a member of the histidine triad superfamily of nucleotide-binding proteins.
Labeling Method: Chemical Labeling, Direct Labeling
Label or Dye: BODIPY™ FL
Product Type: Guanosine 5'-O
Quantity: 50 μL
Shipping Condition: Wet Ice
Concentration: 5 mM
Product Line: BODIPY™
Unit Size: Each