Thermo Fisher, K35920, Vivid Colors™ pcDNA™6.2/C-EmGFP-GW/TOPO™ Mammalian Expression Vector

Vivid Colors™ pcDNA™6.
2/C-EmGFP-GW/TOPO™ Mammalian Expression Vector The Vivid Colors™ pcDNA™6.
2 Fluorescent Protein TOPO™ Expression Vectors (Figure 1) allow you to rapidly clone your gene and fuse it to the widely used and well characterized Fluorescent Proteins (FPs) from the jellyfish Aequorea victoria (1, 2). These powerful TOPO™ cloning vectors contain the Emerald Green Fluorescent Protein (EmGFP) or the Yellow Fluorescent Protein (YFP) for simple, non-invasive detection of recombinant protein (Figure 2). Both FPs have been humanized for optimal mammalian expression (3). The Vivid Colors™ pcDNA™6.
2 Fluorescent Protein TOPO™ Expression Vectors offer:
• Topoisomerase I for one-step, 5-minute TOPO™ cloning of your PCR-amplified gene of interest
• CMV promoter for high-level expression of the recombinant fluorescent fusion protein
• Ability to fuse EmGFP or YFP to the N- or C-terminus of your protein
• Bsd resistance marker for rapid selection of stable cell linesConstitutive or Inducible System: Constitutive
Delivery Type: Transfection
For Use With (Application): Reporter Assays, Subcellular Localization
Product Type: Mammalian Expression Vector
Quantity: 20 reactions
Reporter Gene: GFP (EmGFP)
Selection Agent (Eukaryotic): Blasticidin
Vector: pcDNA
Cloning Method: TOPO-TA
Product Line: Gateway™, TOPO™, Vivid Colors™, pcDNA™
Promoter: CMV
Protein Tag: GFP (EmGFP)
Unit Size: Each