Thermo Fisher, PV3174, Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon a porcine pyruvate liver kinase derived peptide used to study PKA substrate specificity, and containing an R-R-X-S consensus sequence, where X is any residue.
Assay: Serine/Threonine Kinase Assay
Assay Entry: Biochemical phospho-specific activity (no antibody)
Excitation/Emission: 405⁄465, 535
For Use With (Application): FRET
Format: 384-well plate
Label or Dye: Methylcoumarin
No. of Reactions: 800 Reactions
Product Type: Kinase Assay Kit
Quantity: 800 x 20 μL Assays
Readout: End Point
References: BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978)
Sample Type: porcine pyruvate liver kinase (LRRASLG)
Shipping Condition: Dry Ice
Substrate: Peptide-Based Substrate
Target: Serine/Threonine Kinases
Target Entry: PRKX, PRKG2, RPS6KA5, PRKACA, PRKG1, RPS6KA4, AURKB, AURKA
Validated Kinases: AURKA, AURKB, PRKACA, PRKG1, PRKG2, PRKX, RPS6KA4, RPS6KA5
Product Line: Z´-LYTE™
Unit Size: Each