Thermo Fisher, PV3673, Z'-LYTE™ Kinase Assay Kit - Ser/Thr 12 Peptide

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 12 Peptide A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon CDK7, containing Ser-164 and a (S/T)-P-X-(K/R) consensus sequence, where X is any residue.
Assay: Serine/Threonine Kinase Assay
Assay Entry: Biochemical phospho-specific activity (no antibody)
Excitation/Emission: 405/465, 535 nm
For Use With (Application): FRET
Format: 384-well Plate
Label or Dye: Methylcoumarin
No. of Reactions: 800 Reactions
Quantity: 800 x 20 μL Assays
Readout: End Point
References: Mol. Cell. Biol. Vol 21. pp. 88-99 (2001)
Shipping Condition: Dry Ice
Source: Based on CDK7 (GLAKSFGSPNRAY(pT)H)
Substrate Properties: Peptide-Based Substrate
Target Entry: CDK1, CDK2, CDK5
Target Enzyme: Serine/Threonine Kinases
Validated Kinases: CDK1, CDK2, CDK5
Product Line: Z ́-LYTE™
Unit Size: Each