Waters, 176002996, ACQUITY UPLC Protein SEC Column 450 Å, 2.5 µm, 4.6 mm x 150 mm and BEH450 SEC Protein Standard Mix

Based upon the highly successful BEH particle technology, the ACQUITY UPLC BEH450 SEC 2.5 µm column is fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 100,000 to 1,500,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods. Note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.

Specifications
Chemistry: Diol (OH)
Separation Mode: SEC
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 3000 psi (207 Bar)
Endcapped: No
Silanol Activity: Low
Molecular Weight Range Min: 100000
Molecular Weight Range Max: 1500000
Particle Shape: Spherical
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 450 Å
QC Tested: Protein
Format: Column
Packing Solvent: 20% methanol in water
Surface Area: 80
System: UPLC
Particle Technology: BEH
Technique: LC
USP Classification: L33
Inner Diameter: 4.6 mm
Length: 150 mm
Carbon Load: 9 %
eCord: Yes
UNSPSC: 41115709
Application: Protein
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY UPLC Protein SEC Column 450 Å, 2.5 µm, 4.6 mm x 150 mm and BEH450 SEC Protein Standard Mix With this listing, you get access to the ultimate analysis pack with the ACQUITY UPLC Protein BEH SEC Column and BEH450 SEC Protein Standard Mix. This single pack contains both the analytical column and the standard mix that has been designed to benchmark the performance of the column. The ACQUITY UPLC Protein BEH SEC Column has been designed to deliver exceptional improvements in resolution, throughput, and sensitivity for assays. The ACQUITY UPLC Protein SEC Column enables you to monitor the presence of protein aggregates through size-exclusion chromatography (SEC), which results in increased efficacy as the presence of these proteins can compromise the safety and efficacy of biologic-based therapeutics. With the addition of the ACQUITY UPLC Protein BEH SEC Column, you can easily deliver fast, accurate aggregate determinations up to 10 times quicker than traditional HPLC-based SEC methods. The column’s core is designed using the highly successful BEH particle technology and is fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 100,000 to 1,500,000 Daltons. The analytical column is recommended to be used in UPLC SEC applications with an appropriate low LC system volume for optimal SEC separation performance, and it has been optimized for the analysis of proteins and their aggregates to offer robust, fast, and accurate determinations. For optimal column performance of the ACQUITY UPLC Protein BEH SEC Column, utilize the BEH450 SEC Protein Standard Mix to benchmark its performance. The BEH450 SEC Protein Standard Mix contains 4 component protein mixtures, Thyroglobulin, Ovalbumin, Ribonuclease A, and Uracil. We highly recommend the use of this standard periodically for benchmarking the column and your system performance, similar to the QCRM product line. The ACQUITY UPLC Protein BEH SEC Column is based on packing materials that were designed specifically for use with the ACQUITY UPLC System; the column goes through extensive testing and is manufactured in a cGMP, ISO 9001 certified plant using ultra-pure reagents. You can browse through the Waters website to shop for lab equipment and ensure you have all the equipment you need. From the website, you can also review the complete selection of Waters product lines. You may also want to review the ACQUITY UPLC Protein BEH SEC Guard Column, 450 Å, 2.5 µm, 4.6 mm x 30 mm, 1/pk; the ACQUITY UPLC Protein BEH SEC Guard Column, 450 Å, 2.5 µm, 4.6 mm x 30 mm kit contains an advanced, ultra-low volume guard device (4.6 mm ID x 30 mm) containing BEH450, SEC, 2.5 µm particles and guard connection tubing (0.005” x 1.75”).

FAQ
What Is The Carbon Load This Particular ACQUITY UPLC Protein SEC Column Can Support? The ACQUITY UPLC Protein SEC Column can support a carbon load of 9%, along with a particle size of 2.5 µm and featuring pore Size of 450 Å.