Waters, 186005298, ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1/pk

ACQUITY CSH C18 columns were designed to provide alternate selectivity compared to other reversed-phase UPLC columns. These ultra-efficient columns overcome peak shape asymmetry and poor loading capacity for basic compounds, column bleed, and rapid mobile-phase re-equilibration.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 11 pH
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 130 Å
Format: Column
Surface Area: 185
System: UPLC, UHPLC
Particle Technology: CSH
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 150 mm
Carbon Load: 15 %
eCord: Yes
UNSPSC: 41115709
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
FAQs about ACQUITY UPLC CSH C18 Column What distinguishes the ACQUITY UPLC CSH C18 Column in terms of chromatographic performance? The ACQUITY UPLC CSH C18 Column features charged surface hybrid technology that enhances peak shape and resolution for basic compounds, offering excellent chromatographic performance even at low pH levels. Can the ACQUITY UPLC CSH C18 Column be used for the separation of a wide range of compounds? Yes, the ACQUITY UPLC CSH C18 Column is versatile and can be used to separate a broad spectrum of compounds, from small molecules to larger biomolecules, due to its robust design and wide pH stability. How does the particle design of the ACQUITY UPLC CSH C18 Column contribute to UPLC efficiency? The particle design of the ACQUITY UPLC CSH C18 Column, with its small particle size, allows for high-resolution, high-efficiency chromatography, which is ideal for UPLC applications requiring fast analysis times. What are the benefits of the ACQUITY UPLC CSH C18 Column in terms of method development? The ACQUITY UPLC CSH C18 Column's wide pH range and compatibility with a variety of mobile phases make it a valuable tool for method development, offering flexibility to optimize separation conditions for different analytes. Is the ACQUITY UPLC CSH C18 Column compatible with direct injection of biological samples? The ACQUITY UPLC CSH C18 Column is designed to handle complex matrices, including direct injection of biological samples, due to its robust surface technology which provides resistance to sample-matrix effects.

FAQ
What pH range and temperature are recommended for use with the ACQUITY CSH columns? Though designed for a greater lifetime, the column’s lifespan will vary and depend on factors such as the type and concentration of buffer used, and the operating temperature. The use of phosphate buffer at pH 8, combined with higher temperatures will diminish the column’s life. As a general indication, the CSH columns are suited for the analysis of materials with a pH range between 1 and 11.

Up to 80° Celsius temperature is recommended for operating ACQUITY CSH columns in order to enhance selectivity, increase mass transfer rates and lower solvent viscosity. However, when operating at higher pH, lower temperature (45 degrees celsius) is recommended to ensure a longer column lifetime. What is BEH Technology? An instrumental catalyst in UPLC technology, the 1.7 µm BEH (Ethylene Bridged Hybrid) particle serves as a pivotal component, offering diverse pore sizes and bonded phases that facilitate both reversed-phase and hydrophilic interaction chromatography. This cutting-edge technology finds application across a broad spectrum, encompassing the analysis of small molecules as well as large molecule biopharmaceuticals.

Hybrid particle technology has intrinsic chemical stability, allowing for a wider usable pH range [pH 1-12]. This also enables a versatile, robust separation technology for method development.
The BEH particle technology is available in a variety of HPLC particle sizes [2.5, 3.5, 5, and 10 µm] which allows for a seamless transition between HPLC and UPLC technology platforms.