Waters, 186009176, XBridge Protein BEH SEC Column, 200 Å, 2.5 µm, 4.6 mm X 300 mm, 1/pk

XBridge Protein BEH SEC Column, 200 Å, 2.5 µm, 4.6 mm X 300 mm Columns, containing 2.5 µm particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 10,000 to 450,000 Daltons. Each batch is specifically QC tested using Waters BEH200 SEC Protein Standard (p/n: 186006518) to help ensure consistent performance for challenging size-based protein separations. Note: This item is also available with one vial the BEH200 SEC Protein Standard at no additional cost (p/n: 176004336). Suggested Use: Gel Filtration, Size Exclusion Chromatography, Aggregate, Monomer and Fragment analysis If you are developing a new method, please consider our newer technology XBridge Premier Protein 250Å SEC and ACQUITY Premier Protein 250Å SEC Columns for superior recovery, separation, and resolution.

Specifications
Chemistry: SEC
Separation Mode: Gel Filtration (aqueous)
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 6,500 psi (448 Bar)
Endcapped: No
Bonding Technology: Diol (OH)
Silanol Activity: Low
Molecular Weight Range Min: 10000
Molecular Weight Range Max: 450000
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 200 Å
QC Tested: Protein
Format: Online Column
Packing Solvent: 20% methanol in water
Surface Area: 220
System: UHPLC, UPLC, HPLC
Particle Technology: BEH
Technique: LC
USP Classification: L20, L33, L59
Inner Diameter: 4.6 mm
Length: 300 mm
Carbon Load: 12 %
eCord: No
UNSPSC: 41115711
Application: Protein
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge Protein BEH SEC Column, 200 Å, 2.5 µm, 4.6 mm X 300 mm, 1/pk The XBridge Protein SEC BEH Column offers a variety of noteworthy benefits and can handle even the most difficult chromatographic tasks. You will benefit from the column's exceptional mobile-phase pH stability, which contributes to a longer column lifetime. The XBridge Protein SEC BEH Column has great particle efficiency, unrivaled peak form, and capacity, ensuring the robustness of tests. It also boasts excellent column reliability. You will experience an increase in productivity thanks to the thoughtful and deliberate design of the XBridge BEH HPLC Columns. The Columns are versatile and can be used in your laboratory to create cutting-edge LC-MS assays or quality-control procedures. The successful series of HPLC-based SEC columns we offer uses the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as the XBridge Protein BEH SEC, 200 Å Columns, which contain 2.5µm particles. They work best to separate proteins with a dalton range of 10,000 to 450,000. Note: For no additional charge, this item is also available with one vial of the BEH200 SEC Protein Std (PN 176003596). This benchmark should be performed frequently to evaluate the performance of systems and columns. You may easily transition to UPLC Technology by using the XBridge Protein BEH SEC Column. The XBridge Protein BEH SEC Columns are built In cutting-edge Waters specialized Manufacturing facilities. These facilities adhere to stringent industrial procedures to guarantee that the highest quality standards are met and that equipment is built under ISO9001 and cGMP guidelines. Shop for lab equipment and check out our full selection of XBridge columns via the online catalog on our website. If you need help with any equipment or have any questions, don't hesitate to get in touch with a member of our international staff. You may also be interested in checking out the BEH200 SEC Protein Standard Mix; The BEH200 SEC Protein Standard Mix is a 5 component protein mixture (Thyroglobulin, IgG, BSA, Myoglobin, Uracil) used to benchmark ACQUITY UPLC BEH200 SEC, 1.7 µm Columns. It is recommended that this standard be used on a regular basis for benchmarking column and system performance, similar to the QCRM product line.

FAQ
What pH Range Works Best With The XBridge Protein BEH SEC Column? The ideal operating conditions for the XBridge Protein BEH SEC Column include a pH range of 2.5 to 8, a maximum pressure of 6500 psi (or 448 Bar), and a temperature of 60 C. Please be aware that utilizing a higher pH may affect the column's lifespan and functionality.